Among the scientific instructions for the voyage[2] drawn up by a committee of the Royal Society, there is a remarkable letter from Von Humboldt to Lord Minto, then First Lord of the Admiralty, in which, among other things, he dwells upon the significance of the researches into the microscopic composition of rocks, and the discovery of the great share which microscopic organisms take in the formation of the crust of the earth at the present day, made by Ehrenberg in the years 1836-39. Ehrenberg, in fact, had shown that the extensive beds of "rotten-stone" or "Tripoli" which occur in various parts of the world, and notably at Bilin in Bohemia, consisted of accumulations of the silicious cases and skeletons of _Diatomaceæ_, sponges, and _Radiolaria_; he had proved that similar deposits were being formed by _Diatomaceæ_, in the pools of the Thiergarten in Berlin and elsewhere, and had pointed out that, if it were commercially worth while, rotten-stone might be manufactured by a process of diatom-culture. Observations conducted at Cuxhaven, in 1839, had revealed the existence, at the surface of the waters of the Baltic, of living Diatoms and _Radiolaria_ of the same species as those which, in a fossil state, constitute extensive rocks of tertiary age at Caltanisetta, Zante, and Oran, on the shores of the Mediterranean. [2] Of the _Challenger_. Moreover, in the fresh-water rotten-stone beds of Bilin, Ehrenberg had traced out the metamorphosis, effected apparently by the action of percolating water, of the primitively loose and friable deposit of organized particles, in which the silex exists in the hydrated or soluble condition. The silex, in fact, undergoes solution and slow redeposition, until, in ultimate result, the excessively fine-grained sand, each particle of which is a skeleton, becomes converted into a dense opaline stone, with only here and there an indication of an organism. From the consideration of these facts, Ehrenberg, as early as the year 1839, had arrived at the conclusion that rocks, altogether similar to those which constitute a large part of the crust of the earth, must be forming, at the present day, at the bottom of the sea; and he threw out the suggestion that even where no trace of organic structure is to be found in the older rocks, it may have been lost by metamorphosis. CCI