Area 3 has a length of 186 feet, an average width of 34 feet, and a maximum depth of two and one-half feet. This area includes a shallow riffle at both upstream and downstream ends of a pool 73 feet long and approximately one foot in average depth (Pl. 29, Fig. 2). Area 5 has a length of 250 feet, an average width of 50 feet, and a maximum depth of two and one-half feet. This is a shallow, quiet pool over rubble and bedrock bottom except for a small area of mud bottom (backwater) above the point where a short riffle drains into this pool from Area 6 (Pl. 30, Fig. 1). Area 6 has a length of 200 feet, an average width of 50 feet, and a maximum depth of one and one-half feet. This is a shallow, quiet pool over bedrock bottom, except for a small area of mud bottom at one side of the upper end of the pool. A short, steep, rubble-riffle is included in this area at the upstream end (Pl. 30, Fig. 2). Areas 2, 4, and 7 resemble at least one of the areas described above but were sampled less intensively. Data from areas 2, 4, and 7 are included in discussion of the total fauna of the upper Neosho river but are excluded from the discussion of representative parts of that fauna. Methods _Rotenone_ Rotenone was applied to an intermittent pool in 1957. In 1958 and 1959 rotenone was applied to the upper end of a pool and mixed by agitating the water. The concentration in the pool was maintained by slowly introducing part of the rotenone into the riffle at the head of the pool. This was the most effective means of obtaining a large sample of fish from the deeper, slowly flowing water of the upper Neosho. Pools in which rotenone was used had areas of as much as one-half acre and depths in excess of six feet. _Shocker_ In 1959 the shocker was used extensively in several areas of the upper Neosho. Because of the small size of the stream, "tennis-racket" electrodes were used effectively by two men--one carrying the electrodes and one picking up fish and placing them in a live-box. In fast water, many fish floated into a seine placed across the lower end of the area. A large segment of the population was collected in this manner. Areas in which fish were collected by means of the shocker included riffles, and pools having flowing water no more than three feet in maximum depth. The bottom-type was usually gravel, rubble or bedrock, but a small amount of mud bottom was present in many pools. Because of the necessity of wading, we could not use the shocker effectively in water more than three feet deep. In addition, turbidity of the water prevented effective collection of stunned fish in the deeper pools. Therefore, rotenone was more effective in deep water than was the shocker. In shallow, swift riffles and pools, the shocker yielded more reliable samples than did rotenone, because of difficulty in maintaining adequate concentrations of rotenone where flow was swift.