Vegetable carbon employed in the form of charcoal or coke was found to occupy a high place as a biological filter, although previously, owing to its chemical inactivity, it had been disregarded. Being an inexpensive material, and easily renewed, it was destined to be of great service in the purification of water. Experiments were also made by the agitation of water with solid particles. It was found that very porous substances, like coke, animal and vegetable charcoal, were highly efficient in removing organised matter from water when the latter came in contact with them in this manner. Also, it was found that the well-known precipitation process, introduced by Dr. Clark, for softening water with lime, had a most marked effect in removing micro-organisms from water. In the case of water softened by this process, it was found that a reduction of 98 per cent. in the number of micro-organisms was effected, the chemical improvement being comparatively insignificant. Water which had been subjected to an exhaustive process of natural filtration had been found to be almost free from micro-organisms. Thus, the deep-well water obtained from the chalk near London contained as few as eight organisms per cubic centimetre, whereas samples of river water from the Thames, Lea, and Wey had been known to contain as many thousands. The same well-known authority on water published the following statements in the _Nineteenth Century_. He described the subject of domestic filtration as one which, in a town with a water supply like that of London, possesses peculiar interest, and is of no little importance. Most people imagine that by once going to the expense of a filter they have secured for themselves a safeguard which will endure throughout all time without further trouble. No mistake could be greater, for without preserving constant watchfulness, and bestowing great care upon domestic filtration, it is probable that the process will not only entirely fail to purify the water, but will actually render it more impure than before. For the accumulation of putrescent organic matter upon and within the filtering material furnishes a favourable nest for the development of minute worms and other disgusting organisms, which not unfrequently pervade the filtered water; whilst the proportion of organic matter in the effluent water is often considerably greater than that present before filtration.