The following article, first published in the _Popular Science Monthly_, June, 1903, is a report of that test, and indicates a desire to carry the investigation further to include a variety of test-subjects. In response to Professor Chittenden’s request, the Trustees of the Bache Fund of the National Academy of Sciences appropriated $1000 towards a more extended inquiry; and other means having been assured, a project of experiments was taken under consideration. One of the difficulties encountered was the control of test-subjects for a sufficiently long time to make conclusive estimates relative to the minimum needs of nitrogenous food in relation to the common occupations of life. Few if any volunteers, with the leisure and interest fortunately possessed by the writer, were available outside the laboratory force itself, and there were serious objections to using for test-subjects the same persons who did the chemical analyses and estimated the results. In this dilemma the good fortune of a meeting with Surgeon-General O’Reilly of the United States Army and with General Leonard Wood--the former on his way to Madrid to attend a medical congress, and the latter _en route_ to the Philippines to take command there--happened to the writer on the S. S. _Commonwealth_, on a voyage to Italy in April, 1903. Both these officers are medical men and research enthusiasts. They had fought yellow fever together, in coöperation with martyr Dr. Major Walter Reed, in Cuba, and the fame of their success was being talked of as one of the great triumphs of pathologic, or hygienic, science at the time of the meeting. There was ample time on the steamer to discuss a subject of such mutual and general interest, and both officers had had, in service, experiences that led them to believe that the results obtained by the writer and his colleagues were the common possibilities of all persons under right conditions of alimentation. General O’Reilly was of the opinion that the corps he commanded could furnish intelligent and earnest test-subjects for nutrition investigation, as it did in the yellow fever case. In the yellow fever investigation privates and officers alike had volunteered to act as test-subjects, even though their lives were at stake and many had already been sacrificed. It is to their great honour, also, that they refused to receive the bounty that was offered for test-subjects, preferring to serve science and humanity freely as volunteers rather than sell themselves as experimental risks. From such material General O’Reilly was sure that capable assistants could be secured to test the not at all dangerous or disagreeable economies of nutrition that the projected inquiry wished to solve.