The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
It has been before stated that some of the zoophytes may be mounted
dry, and others examined as opaque or transparent objects according to
their substance. They are very interesting when examined in the trough
whilst living, but to preserve many of them for future examination they
must be mounted in some preservative medium. Sometimes this may be one
of the liquids mentioned in Chapter IV., but if possible they should
be kept in balsam, as there is less danger of injury by accident to
this kind of slide. This method of mounting presents some difficulties,
but I think that all agree as to the trustworthiness of Dr. Golding
Bird’s information on the subject, which appeared in the _Microscopic
Journal_. Of this, space forbids me to give more than a condensed
account, but I hope to omit nothing of moment to the reader for whom
these pages are written.
After stating that there are few who are not familiar with these
exquisite forms, and have not regretted the great loss of beauty
they sustain in dying, he informs us that from their so obstinately
retaining air in the cells and tubes when dried, it is hardly
practicable to get rid of it; and they also shrivel up very seriously
in the process of drying. The following plan, however, he has found
almost faultless in their preparation.
To preserve them with extended tentacles, they should be plunged in
cold fresh water, which kills them so quickly that these are not often
retracted. The specimens should be preserved in spirit until there is
leisure to prepare them; if, however, they have been _dried_, they
should be soaked in cold water for a day or two before being submitted
to the following processes:--
1. After selecting perfect specimens of suitable size, immerse them
in water heated to about 120° and place them under the receiver of an
air-pump. Slowly exhaust the air, when bubbles will rise and the water
appear to be in a state of active ebullition. After a few minutes
re-admit the air and again exhaust, repeating the process three or four
times. This will displace the air from most, if not all, of the class.
2. Remove the specimens and allow them to drain upon blotting-paper
for a few seconds; then place them in an earthen vessel fitted with a
cover, and previously heated to about 200°. This heat may be easily
got by placing the vessel for a short time in boiling water, wiping
it immediately before use with a thick cloth. The specimens are then
dropped into this, covered with the lid, and immediately placed under
the receiver of the air-pump, and the air rapidly exhausted. By this
means they are dried completely, and so quickly that the cells have no
time to wrinkle.
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