The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and development — John Shaqi
The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and developmentEaland, C. A. (Charles Aubrey)
History
The Romance of the Microscope: An interesting description of its uses in all branches of science, industry, agriculture, and in the detection of crime, with a short account of its origin, history, and development
Ealand, C. A. (Charles Aubrey)
Microscopes; Microscopy
We shall see this green colouring matter over and over again in our
botanical studies, in fact it is found in all manner of situations,
in leaf and stem. Very often its colour is hidden by sap of another
colour, as for instance in copper beech leaves or in the brown
seaweeds. Chlorophyll dissolves in alcohol, however, and this affords
us a ready means of detecting its presence though we cannot see its
green colour. If we boil any leaf, suspected of containing chlorophyll,
in alcohol we shall obtain a solution with rather peculiar properties
because, when held up to the light it appears green, but when light is
reflected from it, it appears reddish.
From the under side of the cabbage leaf whence we obtained our first
specimen of chlorophyll, we must now take another piece of skin. If
we perform the operation properly the skin will be colourless, like
a piece of thin parchment; any green colour will show that we have
torn off more than the skin and we must make another attempt. Having
secured our piece of skin we place it in a drop of water on a clean
slide and examine it under the microscope. We first notice that the
skin is divided up into a number of small areas called cells and dotted
here and there amongst the cells are several oval bodies, containing
chlorophyll. These oval bodies are the pores through which the leaf
breathes, amongst other things. In the centre of each pore there is
a hole, at least there is if the pore is open, for the two cells
comprising the pore have the power of opening and closing.
It is interesting to try the same experiment with a fern leaf and to
notice that there are pores, very similar to those of the cabbage, but
that the walls bounding the cells of the leaf are irregular and that
they contain chlorophyll. We may try several other leaves and also the
upper and lower surfaces of leaves, then we shall soon learn that, in
leaves with distinct upper and lower surfaces, there are far more pores
on the lower than the upper surface; leaves like those of the iris have
almost the same number on each side, and floating leaves, like those of
water lily have all their pores on the upper side. There is a reason
for this; the pores are likely to become filled with dust, being on the
lower side they are protected somewhat; flat leaves, by their shape,
afford no protection and floating leaves must have their pores on the
upper surface to obtain air.
Public-domain text, read in full here on John Shaqi.
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