Half a century was to elapse before there was another case involving the episcopal Order. It has been sometimes thought that the Inquisition was concerned in the trial and execution of Antonio de Acuña, Bishop of Zamora, but such was not the fact, although the case illustrates the difficulty of holding a bishop accountable for his misdeeds. That turbulent prelate, somewhat absurdly styled a second Luther by Leo X, was an active leader in the Comunidades, who, after the defeat at Villalar, April 21, 1521, fled in disguise but was caught at Villamediana, on the Castilian border. Episcopal immunity rendered him a doubtful prize; Charles V was resolved on his death, but there was considerable doubt as to how he was to be punished. The Inquisition was not brought into play but, after some negotiation, Leo X was induced to issue a commission to Cardinal Adrian and the nuncio to take testimony and forward it for judgement by the pope in consistory. On Adrian's accession to the papacy he transferred the commission to the Archbishop of Granada and the Bishop of Ciudad-Rodrigo, but gave no authority to employ torture. Then Clement VII, by a brief of March 27, 1524, granted faculties to proceed to extremities, under which the trial went on, but apparently died out when carried to Rome. Wearied with five years' confinement in the castle of Simancas, Acuña made a fruitless attempt to escape, in which he killed the alcaide, Mendo Noguerol. Charles then sent to Simancas his alcalde de casa y corte, Rodrigo Ronquillo, with instructions to torture Acuña and put him to death--instructions faithfully executed, March 23, 1526. This violation of the immunities of the Church caused no little scandal. Charles speedily obtained for himself, from Clement, absolution from the _ipso facto_ excommunication incurred, but that which he had promised to procure for his subordinates was granted with difficulty and only after delay of more than a year, the final ceremony not taking place until September 8, 1527. At Valladolid a tradition was long current that Ronquillo came to an evil end, being carried off by demons.[130] As the Lutheran revolt grew more threatening and the dread of its extending to Spain increased, a certain limited jurisdiction over bishops was conferred on Cardinal Manrique by a brief of Clement VII, July 15, 1531. He was empowered to inquire against them if suspected of favoring Lutheran doctrines or of aiding those who held them; he was not permitted, however, to arrest and imprison, although he could punish them according to the canons and he was granted the fullest faculties of absolving and rehabilitating those who abandoned their errors and asked for forgiveness.[131] It is not likely that any occasion arose for the exercise of these faculties, but if there was it has left no trace.