Mix forty parts of (1) and one part of (2). In a test-tube take equal parts of this mixture and the urine, and pour 1 or 2 c.c. of the ammonia upon its surface. If the reaction be positive, a garnet ring will form at the junction of the two fluids; and upon shaking, a distinct pink color will be imparted to the foam. The color of the foam is the essential feature. If desired, the mixture may be well shaken before the ammonia is added: the pink color will then instantly appear in that portion of the foam which the ammonia has reached, and can be readily seen. The color varies from eosin-pink to deep crimson, depending upon the intensity of the reaction. A doubtful reaction should be considered negative. * * * * * 7. Pancreatic Reaction.--Cammidge has shown that in cases of pancreatitis a substance capable of forming {92} crystals with phenylhydrazin can be developed by boiling the urine with a mineral acid, and has offered the following test as an aid in diagnosis of this obscure condition. The nature both of this substance and the antecedent substance from which it is derived is not known. As originally proposed, the test was complicated and probably not trustworthy, but with his improved and simplified technic, Cammidge has had very promising results. In 200 consecutive examinations in which the diagnosis was confirmed, _postmortem_ or at operation, 67 cases of pancreatitis (65 chronic, 2 acute) gave positive reactions; 4 cases of cancer of the pancreas were positive, 12 negative; 4 cases in which no pancreatitis was found were positive, 113 were negative. Normal urines do not give the reaction. The difficulty and importance of diagnosis in pancreatitis warrant inclusion of the method here even though its true value cannot be definitely assigned. While the test is somewhat tedious, all the manipulations are simple and require no apparatus but flasks, test-tubes, and funnels. * * * * * Technic.--Careful attention to detail is imperative. An ordinary routine examination is first made. Albumin and sugar, if present, must be removed: the former, by acidifying with acetic acid, boiling, and filtering; the latter, by fermentation with yeast after the first step of the method proper. An alkaline urine should be made slightly acid with hydrochloric acid.