Law of Substitution.--By the careful study of the action of Chlorine, and of Nitric Acid, upon various organic substances, a remarkable series of compounds has been discovered, containing a portion of Chlorine or of Peroxide of Nitrogen in the place of Hydrogen. The peculiarity of these substances is, that they strongly resemble the originals in their physical, and often in their chemical properties. It might have been supposed that agents of such active chemical affinities as Chlorine and Oxide of Nitrogen would, by their mere presence in a body, produce a marked effect; yet it is not so in the case before us. The primitive type or constitution of the substance modified remains the same, even the crystalline form being often unaffected. It seems as if the body by which the Hydrogen had been displaced had stepped in quietly and taken up its position in the framework of the whole without disturbance. Many compounds of this kind are known; they are termed by chemists "substitution compounds." The law invariably observed is, that the substitution takes place in equal atoms: a single atom of Chlorine, for instance, displaces one of Hydrogen; two of Chlorine displace two of Hydrogen, and so on, until, in some cases, the whole of the latter element is separated. In illustration of these remarks, take the following instances:--Acetic Acid contains Carbon, Hydrogen, and Oxygen; by the action of Chlorine the Hydrogen may be removed in the form of Hydrochloric Acid, and an equal number of atoms of Chlorine be substituted. In this way a new compound is formed, termed _Chloracetic Acid_, resembling in many important particulars the Acetic Acid itself. Notice particularly that the peculiar properties characteristic of Chlorine are completely masked in the substitution body, and no indication of its presence is obtained by the usual tests! A soluble _Chloride_ gives with Nitrate of Silver a white precipitate of Chloride of Silver, unaffected by Acids, but the Chloracetic Acid does not; hence it is plain that the Chlorine exists in a peculiar and Ultimate state of combination different from what is usual. The substance we have been previously considering, viz. Pyroxyline, affords another illustration of the Law of Substitution. Omitting, for the sake of simplicity, the number of atoms concerned in the change, the action of concentrated Nitric Acid upon ligneous fibre may be thus explained:-- { Carbon Cotton _or_ { Hydrogen + Nitric Acid { Hydrogen { Oxygen equals { Carbon Pyroxyline _or_ { Hydrogen + Water { Peroxide Nitrogen { Oxygen Or in symbols:-- CH{11}O + NO{5} = C (H{n-1}NO{4}) O + HO