Microscopic analysis of plant epidermis is useful in helping to
determine food habits of various animals (Dusi, 1949; Williams, 1955,
1959a; Brusven and Mulkern, 1960; Johnson, 1962). The microscopic
analysis of stomach contents provides a practical method of determining
which plants are eaten by rodents. Contents of stomachs and intestines
were removed from mice caught in snap traps, and from preserved
specimens. The contents were placed on a piece of bolting silk, washed
thoroughly with running water, stained with iron-hematoxylin and mounted
on slides, or stored in 70 per cent ethanol (Williams, 1959a; Douglas,
1965).
TABLE 6--Plants, or Parts of Plants, Eaten by Captive Individuals of
_P. maniculatus_ in Mesa Verde National Park, Colorado. 0 = not
eaten, + = eaten, - = not offered.
=============================+========+======+========+=======
Species of Plant | Leaves | Stem | Flower | Seeds
-----------------------------+--------+------+--------+-------
_Artemisia ludoviciana_ | 0 | 0 | - | -
_Calochortus gunnisonii_ | 0 | + | - | +
_Chaenactis douglasii_ | 0 | 0 | - | -
_Comandra umbellata_ | + | + | - | -
_Erigeron speciosus_ | 0 | 0 | - | -
_Eriogonum alatum_ | - | - | - | +
_Juniperus osteosperma_ | - | - | - | +
_Lappula redowskii_ | 0 | 0 | - | +
_Lithospermum ruderale_ | 0 | 0 | - | 0
_Orthocarpus purpureo-albus_ | 0 | 0 | + | +
_Penstemon linarioides_ | + | + | + | -
_Pinus edulis_ | - | - | - | +
_Purshia tridentata_ | + | + | - | -
_Sitanion hystrix_ | 0 | 0 | - | 0
_Solidago petradoria_ | 0 | 0 | 0 | -
_Sphaeralcea coccinea_ | + | + | - | +
_Stipa comata_ | 0 | 0 | - | +
_Viguiera multiflora_ | + | + | - | -
-----------------------------+--------+------+--------+-------
In order to analyze these epidermal fragments, a collection of plants
was made within the park. Slides of the epidermis of these plants were
prepared and analyzed for diagnostic characters (Douglas, 1965:197-199).
Features such as the stomatal arrangement in relation to subsidiary
cells; the types of trichomes, scales and glands; the cellular
inclusions such as starch grains, mucilage and resins are of taxonomic
value (Metcalfe and Chalk, 1950). The configuration of the anticlinal
cell walls is useful in separating species that are similar in other
respects (Douglas, 1965:199).
The following species of plants, and other food items, were identified
in the stomach or intestinal contents of _Peromyscus maniculatus_:
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