It is said that the Physiological method is especially _comparative_[4]; and this dictum also finds favour in the eyes of many. I should be sorry to suggest that the speculators on scientific classification have been misled by the accident of the name of one leading branch of Biology--_Comparative Anatomy_; but I would ask whether _comparison_, and that classification which is the result of comparison, are not the essence of every science whatsoever? How is it possible to discover a relation of cause and effect of _any_ kind without comparing a series of cases together in which the supposed cause and effect occur singly, or combined? So far from comparison being in any way peculiar to Biological science, it is, I think, the essence of every science. A speculative philosopher again tells us that the Biological sciences are distinguished by being sciences of observation and not of experiment![5] Of all the strange assertions into which speculation without practical acquaintance with a subject may lead even an able man, I think this is the very strangest. Physiology not an experimental science! Why, there is not a function of a single organ in the body which has not been determined wholly and solely by experiment. How did Harvey determine the nature of the circulation, except by experiment? How did Sir Charles Bell determine the functions of the roots of the spinal nerves, save by experiment? How do we know the use of a nerve at all, except by experiment? Nay, how do you know even that your eye is your seeing apparatus, unless you make the experiment of shutting it; or that your ear is your hearing apparatus, unless you close it up and thereby discover that you become deaf? It would really be much more true to say that Physiology is _the_ experimental science _par excellence_ of all sciences; that in which there is least to be learnt by mere observation, and that which affords the greatest field for the exercise of those faculties which characterise the experimental philosopher. I confess, if any one were to ask me for a model application of the logic of experiment, I should know no better work to put into his hands than Bernard's late Researches on the Functions of the Liver.[6] Not to give this lecture a too controversial tone, however, I must only advert to one more doctrine, held by a thinker of our own age and country, whose opinions are worthy of all respect. It is, that the Biological sciences differ from all others, inasmuch as in _them_ classification takes place by type and not by definition.[7]