_Exclusion of the factor of Excitability._--The object of the enquiry being the pure effect of variation of conductivity, we have to assure ourselves that under the particular conditions of the experiment the complicating factor of polar variation of excitability is eliminated. It is to be remembered that excitatory transmission in _Mimosa_ takes place by means of a certain conducting strand of tissue which runs through the stem and the petiole. In the experiment to be described, the constant current enters by the tip of the petiole and leaves by the stem, or _vice versâ_, the length of the intrapolar region being 95 mm. The point of application of stimulus on the petiole is 40 mm. from the electrode at the tip of the leaf. The responding pulvinus is also at the same distance from the electrode on the stem. The point of stimulation and region of response are thus at the relatively great distance of 40 mm. from either the anode or the cathode, and may therefore be regarded as situated in the indifferent region. This is found to be verified in actual experiments. EFFECTS OF DIRECTION OF CURRENT ON VELOCITY OF TRANSMISSION. A very convincing method of demonstrating the influence of electric current on conductivity consists in the determination of changes induced in the velocity of transmission by the directive action of the current. For this purpose we have to find out the true time required by the excitation to travel through a given length of the conducting tissue (1) in the absence of the current, (2) ‘against’ and (3) ‘with’ the direction of the current. The true time is obtained by subtracting the latent period of the pulvinus from the observed interval between the stimulus and response. Now the latent period may not remain constant, but undergo change under the action of the polarising current. It has been shown that the excitability of the pulvinus does not undergo any change when it is situated in the middle or indifferent region. The following results show that under parallel conditions the latent period also remains unaffected:-- TABLE V.--SHOWING THE EFFECT OF ELECTRIC CURRENT ON THE LATENT PERIOD. +------------------------------------------------+-------+-------+ | Specimens | I. | II. | |------------------------------------------------+-------|-------| | | sec. | sec. | | Latent period under normal condition | 0.10 | 0.09 | | " " current from right to left | 0.11 | 0.10 | | " " current from left to right | 0.09 | 0.09 | +------------------------------------------------+-------+-------+