Another property of juglone is its toxicity to fish. A few years ago it was a common practice in the South to cut the husks from young nuts and throw them immediately into a still pond of water. The fish, stunned by the juglone, would rise to the surface and were collected and eaten. No one seemed to worry about the effects of such poisoned food on the consumers. Juglone is toxic to fungi and bacteria. Of all the medicinal powers attributed to walnuts by the Greeks and Romans, its use in curing certain skin diseases including ringworm has held up through the ages until many today can recall the use of the green husks for control of ringworms. Brissemoret and Michaud (1917) reported the use of juglone in clinical cases for the cure of eczema, psoriasis, impetigo and other skin diseases and concluded that juglone deserves extensive use in dermatology. To our knowledge the medical profession has not followed up the possibilities which this substance offers. The author is familiar with one case in which pure juglone was applied to a persistent ringworm infection. The infection disappeared within a month after treatment was begun. Though conclusions can not be drawn on a single case, certainly this observation lends credence to the medicinal lore of the ancients and the American pioneers. During the fall and winter of 1942-43, investigations on juglone were started at the Connecticut Agricultural Experiment Station in conjunction with studies of the effect of other plant toxins on the roots of higher plants. When the toxicity of this oxidizing compound was established, it was produced in some quantity both by extraction from walnuts after the method of Combes (1907) and by synthesis after the method of Bernthsen and Semper (1887). Working on the assumption that the killing of germinating fungus spores and root hairs are similar phenomena, juglone was subjected to standardized laboratory tests for fungicidal value. In a series of experiments, this compound proved to be equally toxic with the copper in Bordeaux mixture. Such a high degree of toxicity was deemed worth further investigation, so juglone was tested as a seed protectant and as a spray in field trials for the control of black spot of roses. As a seed protectant, juglone failed miserably. It's toxicity to the noncutinized surfaces of root tissues was so great that germination was abnormal and greatly impaired. The injury noted here was apparently the same as that discussed by Brown (1943) and that which occurs normally in the field. In field tests on the control of black spot of roses juglone stood up well. No phytotoxic activity could be noted on the cutinized stem and leaf surfaces. On the variety George Ahrens, juglone gave equal control with 2-1/2 times as much 325 mesh sulfur, the standard control for this disease. SUMMARY 1. Under certain conditions walnut trees exhibit toxicity to those plants whose roots are in intimate contact with the roots of the walnut.