Physiology and histology of the Cubomedusæ: including Dr. F.S. Conant's notes on the physiologyBerger, E. W. (Edward William)
Science
Physiology and histology of the Cubomedusæ: including Dr. F.S. Conant's notes on the physiology
Berger, E. W. (Edward William)
Cubomedusae
_Lithocyst and Concretion._--The cavity filled by the concretion is
lined in places by a single layer of cells, two of which are shown in
Fig. 7. This fact has been noted by both H. V. Wilson and Conant. Such
cells are evidently remnants of the cells that formed the concretion. The
supporting lamella completely surrounds the cavity of the concretion.
The concretion filling the lithocyst has the shape of a hemiprolate
spheroid cut in the plane of the axis of revolution. Whether it is of
endo- or of ectodermal origin, I believe developmental studies only can
determine. Tests made in the Chemical Laboratory show the presence of
calcium sulphate with perhaps a very small trace of phosphate.[i] Nitric
acid slowly dissolves these concretions, but I believe Claus was mistaken
when he said that they dissolve with an evolution of gas. I watched
them dissolve under the microscope, and never could see the least bit
of gas formed. If Claus’s observation is correct, then the composition
of the concretions of C. marsupialis is different from that of the
concretions of C. Xaymacana. The concretions, further, were dissolved out
of the material preserved in formaline and in osmic acid solutions. For
dissolving them in situ I used either nitric or hydrochloric acid, or
both. A slight husk remains after all the lime is dissolved.
_The Epithelium of the Clubs._--The epithelium is thickest on the dorsal
side of a club. The thickening here, as in several other places, seems
to be due to a crowding of the cells, in consequence of which the nuclei
come to lie at different levels, but I believe that all the cells quite
reach the surface. The cells with their nuclei nearest the surface are
pyramidal in shape, with the bases of the pyramids toward the surface,
while those cells whose nuclei lie deeper (where several layers of nuclei
occur) may be spindle-shaped (Figs. 12, 23, 24, 26). Centrad these cells
are continued into a single process, which often seems to extend to the
basement membrane (Figs. 7, 12, 13, 23, 24). Where the epithelium covers
the region of the concretion, the cells become flattened and with the
long axis of their nuclei parallel with the surface of the club (Fig. 7).
The same holds true for the corneal epithelium (Figs. 7, 13).
It is a significant fact that in many places the nuclei form only a
single layer, and in such places one cannot speak of spindle-shaped
cells. I cannot find any evidence of sensory and supporting cells as
Schewiakoff describes. The fact that spindle-shaped cells may exist is
simply a physical consequence of their being closely crowded. Conant
arrived at the same conclusion.
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