Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal SectionsMarsh, Sylvester
Science
Section-Cutting: A Practical Guide to the Preparation and Mounting of Sections for the Microscope, Special Prominence Being given to the Subject of Animal Sections
Marsh, Sylvester
Microscopy; Microscopy -- Technique
To merely mention the substitutes for Turkey oilstone would fill some
pages. We therefore confine ourselves to the German hone. It is obtained
from the slate mountains in the neighborhood of Ratisbon, where it
occurs in the form of a yellow vein running vertically into the blue
slate, sometimes not more than an inch in thickness, and varying to
twelve to eighteen inches. After being quarried it is sawed into thin
slabs, which are usually cemented to a similar slab of the blue slate as
a support, and the combined stones are then set in a wooden frame and
fitted with a cover, also of wood. Unlike the Turkey oilstone, the
Arkansas stone, and some others, the German hone is soft and easily
scratched and worn. It must therefore be carefully preserved, and as the
_flatness_ of the hone is an important point, it should never be used
for anything but section knives.
Having purchased a hone, the first thing to do is to see that it is
perfectly true; that is, that its surface is a perfect plane. If hollow
or twisted, it is useless until made straight. It may be tested by means
of two steel or wood straight-edges, and the method of doing this, which
is very simple but difficult to describe, the microscopist can learn
from any cabinet maker or carpenter. If the hone be not true it must be
made so, and this is best done by grinding it with emery on a slab of
marble or, better still, a plate of cast iron which has been planed
true. (Waste castings with planed surfaces may be found in most machine
shops, and may be either borrowed or purchased for a small sum.) If the
hone is very uneven, coarse emery may be used at first; then finer must
be taken, and so on until the surface of the hone is not only true but
very smooth. This involves considerable labor, but it cannot be avoided
if a _true_ edge as well as a sharp one is desired, and hence our advice
to use the hone for nothing but section knives. Accuracy in a penknife
or a razor for shaving is of no consequence; in a section-knife it is a
_sine qua non_.
The surface of the hone must not only be true and smooth, it must also
be clean and free from dust and grit, a single particle of which may
spoil the work of hours. It should therefore, when not in use, be kept
constantly covered. It is always used with oil, and this oil should be
of a kind that does not readily dry up. Good sperm is excellent, and so
is purified neat’s-foot oil. Avoid kerosene, soap and water, and similar
fluids, which are so frequently recommended, especially under the guise
of new discoveries. They have all been tried and found wanting. After
use, the stone should be carefully wiped clean, fresh oil being applied
when it is next used. It must never be used without a liberal supply of
oil, otherwise it will become glazed and will no longer act on the
steel.
Public-domain text, read in full here on John Shaqi.
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