(_b_) Manual exploration of the principal arteries for pulsation or thrill. (_c_) Magnus‘s test, applying a ligature tightly round a finger, sufficient to stop the venous but not the arterial circulation, and noticing whether or not a bloodless ring forms at the seat of ligature, and a zone of livid redness on the distal side of the ligature, the part becoming first red, then purple. (_d_) Applying pressure to the finger-nail, and noticing whether the colour disappears on pressure, and a pink tinge appears after relaxing the pressure. (_e_) Applying heat, such as dropping melted sealing-wax on the skin, and noting whether or not redness or vesication ensues. (_f_) Holding the hand, with the fingers abducted, against a strong fight, and observing whether or not the web of the fingers is translucent. (_g_) Inserting a brightly polished needle into a fleshy part of the body, allowing it to remain for ten seconds or so _in situ_, and noticing whether it is tarnished or not on withdrawing it. (_h_) Injecting hypodermically a solution of fluorescin (resorcin-phthalein and sodium bicarbonate, a gramme of each dissolved in 8 c.c. of water). No local discoloration of the skin takes place if the circulation has ceased, but if not, a yellowish-green coloration of the skin occurs round the seat of injection, and the substance may be detected in the blood at a part some distance from the seat of injection. By immersing some white silk threads in the blood drawn at a distance from the prick, then boiling them in distilled water, the latter will have a greenish colour if the fluorescin has been circulated (Icard‘s test). These tests will detect whether the circulation has ceased or not, and so differentiate suspended animation from real death. 2. The _lustre of the eye_ is lost immediately after death. It has, however, been stated that the iris will respond to the action of atropine and eserine for some hours after death, and that the action of the latter is always more marked than that of the former. The fundus as seen by ophthalmoscopic examination is altered, the normal redness changes to a yellowish-white, the vessels in the disc and just around it become empty, and the veins appear to contain bubbles of gas and the column of blood is broken up (Bouchet). A blackish round or oval stain has been described by M. Larcher on the sclerotic coat on the outer side, which he calls _l‘imbibition cadavérique du fond de l‘œil_. It is probably due to thinning of the sclerotic from evaporation, enabling the choroid to be seen through it. The spot precedes rigidity and is a forerunner of putrefaction. 3. The _most powerful stimulus applied to the body does not cause any reaction_. The muscles _may_, however, be made to contract shortly after death by the stimulus of a slight blow, or by galvanism. 4. _The surface of the body becomes of an ashy-white colour._ Exceptions.