The Fundamentals of BacteriologyMorrey, Charles Bradfield
Science
The Fundamentals of Bacteriology
Morrey, Charles Bradfield
Bacteriology
Phosphorus is present especially in the nucleoproteins and nucleins.
Just what the intermediate stages are, on whether there are any, so
far as the phosphorus is concerned, in the splitting up of nucleic
acid by bacterial action is not determined. The phosphorus may occur
as phosphoric acid in such decompositions, or when the conditions are
anaërobic, as phosphine (PH₃), which burns spontaneously in the air to
phosphorus pentoxide (P₂O₅), and water.[13]
The hydrogen in proteins appears in the forms above indicated: H₄C,
H₃N, H₃P, H₂S, H₂O and as free H. The oxygen as CO₂ and H₂O.
In the breaking down of the complex protein molecule even by a single
kind of bacterium there is not a perfect descending scale of complexity
as might be supposed from the statement that there result proteoses,
peptones, polypeptids, amino-acids. These substances do result, but at
the time of their formation simpler ones are formed also, even CO₂,
NH₃ and H₂S. It appears that the entire molecule is shattered in such
a way that less complex proteins are formed from the major part, while
a minor portion breaks up completely to the simplest combinations
possible. A more complete knowledge of these decompositions will aid
in the further unravelling of the structure of proteins. The presence
or absence of free oxygen makes a difference in the end-products, as
has been indicated. There are bacteria which oxidize the ammonia to
nitric acid and the H₂S to sulphuric acid. (See Oxidation, Chapter XI.)
Bacteria which directly oxidize phosphorus compounds to phosphoric
acid have not been described. It does not seem that such are necessary
since this is either split off from nucleic acid or results from the
spontaneous oxidation of phosphine when this is formed under anaërobic
conditions.
Not only are proteins decomposed as above outlined, but also their
waste products, that is, the form in which their nitrogen leaves the
animal body. This is largely urea in mammals, with much hippuric acid
in herbivorous animals and uric acid in birds and reptiles. These
substances yield NH₃, CO₂ and H₂O with a variety of organic acids
as intermediate products in some cases. The strong odor of ammonia
in stables and about manure piles is the everyday evidence of this
decomposition.
Where the putrefaction of proteins occurs in the soil with moderate
amounts of moisture and free access of air a large part of the products
is retained in the soil. Thus the ammonia and carbon dioxide in the
presence of water form ammonium carbonate; the nitric, sulphuric and
phosphoric acids unite with some of the metals which are always present
to form salts. Some of the gases do escape and most where the oxygen
supply is least, since they are not oxidized.
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