The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
Lastly, it should also be noted that it is numerical and not angular
aperture which measures the quantity of light admitted to the objective
by different pencils.
[Illustration: Fig. 38.]
[Illustration: Fig. 38_a_.]
First take the case of the medium being the same. The popular notion
of a pencil of light may be illustrated by Fig. 38, which assumes that
there is equal intensity of emission in all directions, so that the
quantity of light contained in any given pencils may be compared by
simply comparing the contents of the solid cones. The Bouguer-Lambert
law, however, shows that the quantity of light emitted by any bright
point varies with the obliquity of the direction of emission, being
_greater_ in a perpendicular than in an oblique direction. The rays are
less intense in proportion as they are more inclined to the surface
which emits them, so that a pencil is not correctly represented by Fig.
38, but by Fig. 38_a_, the density of the rays decreasing continuously
from the vertical to the horizontal, and the squares of the sines of
the semi-angles (_i.e._, of the numerical aperture) constituting the
true measure of the quantity of light contained in any solid pencil.
If, again, the media are of different refractive indices, as air
(1·0), water (1·33), and oil (1·52), the total amount of light emitted
over the whole 180° from radiant points in these media under a given
illumination is not the same, but is _greater_ in the case of the
media of greater refractive indices in the ratio of the squares of
those indices (_i.e._, as 1·0, 1·77 and 2·25). The quantity of light
in pencils of different angle and in different media must therefore
be compared by squaring the product of the sines and the refractive
indices, _i.e._ (_n_ Sin _u_^2), for the square of the numerical
aperture.
The fact is therefore made clear that the aperture of a dry objective
of 180° does not represent, as was supposed, a maximum, but that
aperture increases with the increase in the refractive index of the
immersion fluid; and it should be borne in mind that this result has
been arrived at in strict accordance with the ordinary propositions of
geometrical optics, and without any reference to or deductions from the
diffraction theory of Professor Abbe.
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