The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
The whole aperture question, notwithstanding the innumerable
perplexities which heretofore surrounded it, is in reality completely
solved by these two simple considerations: First, that “aperture” is
to be applied in its ordinary meaning as representing the greater or
less capacity of the objective for receiving and transmitting rays;
and second, that when so applied the aperture of an objective is
determined by the ratio between its opening and its focal length; the
objective that utilises the larger back lens (or opening) relatively
to its focal length having necessarily the larger aperture. It would
hardly, therefore, serve any useful purpose if we were here to discuss
the various erroneous ideas that gave rise to the contention that 180°
in air must be the maximum aperture. Amongst these was the suggestion
that the larger emergent beams of immersion objectives were due to
the fact that the immersion fluid abolished the refractive action of
the first plane surface which, in the case of air, prevented there
being any pencil exceeding 82° within the glass. Also the very curious
mistake which arose from the assumption that a hemisphere did not
magnify an object at its centre because the rays passed through without
refraction. A further erroneous view has, however, been so widespread
that it seems to be desirable to devote a few lines to it, especially
as it always appears at first sight to be both simple and conclusive.
[Illustration: Fig. 37.]
[Illustration: Fig. 37_a_.]
If a dry objective is used upon an object in air, as in Fig. 37, the
angle may approach 180°, but when the object is mounted in balsam,
as in Fig. 37_a_, the angle at the object cannot exceed 82°, all
rays outside that limit (shown by dotted lines) being reflected back
at the cover-glass and not emerging into air. On using an immersion
objective, however, the immersion fluid which replaces the air above
the cover-glass allows the rays formerly reflected back to pass through
to the objective, so that the angle at the object may again be nearly
180° as with the dry lens. The action of the immersion objective was,
therefore, supposed to be simply that it repaired the loss in angle
which was occasioned when the object was transferred from air to
balsam, and merely restored the conditions existing in Fig. 37_a_ with
the dry objective on a dry object.
As the result of this erroneous supposition, it followed that an
immersion objective could have no advantage over a dry objective,
except in the case of the latter being used upon a balsam-mounted
object, its aperture then being (as was supposed) “cut down.” The
error lies simply in overlooking the fact that the rays which are
reflected back when the object is mounted in balsam (Fig. 37_a_) are
not rays which are found when the object is in air (Fig. 37), but are
_additional and different_ rays which do not exist in air, as they
cannot be emitted in a substance of so low a refractive index.
Public-domain text, read in full here on John Shaqi.
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