The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
=Measure of Apertures of Objectives. N.A.=--Numerical aperture, as it
is termed, is measured by the scale of measurement calculated by the
late Professor Abbe, and which has since been generally recognised and
adopted. He showed that even in lenses made for the same medium (as
air) their comparative aperture as compared with their focus was not
correctly measured by the angle of the rays grasped, but by the actual
diameters of the pencil of rays transmitted, which depend, as already
seen, more upon the back of the lens than the front. To get a geometric
measure for comparison, he took the radii, or half diameters (whose
relative proportions would be the same), and which geometrically are
the sines of the semi-angle of the outermost rays grasped. Abbe further
showed that if this sine of half the outside angle were multiplied by
the refractive index of the medium used we should have a number which
would give the comparative _aperture_ of any lens, whatever the medium.
This number, then, determines both the numerical aperture and the
resolving power of the objective.
The following table of numerical apertures shows the respective angular
pencils which they express in air, water and cedar oil, or glass.[16]
The first column gives the numerical apertures from 0·20 to 1·33; the
second, third, and fourth, the air, water and oil (or balsam) angles of
aperture from 23° 4′ air angle to 180° balsam angle. The theoretical
resolving power in lines to the inch is shown in the sixth column; the
line E of the spectrum being taken from about the middle of the green,
the column giving “illuminating power” being of less importance; while
in using that of penetrating power, it must be remembered that several
data beside that of 1/_a_ go to make up the total depth of vision with
the microscope.
ABRIDGED NUMERICAL APERTURE TABLE.
Public-domain text, read in full here on John Shaqi.
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