[62] The results of this experiment are most interesting, especially if compared with what takes place when some of the same fluid is neutralized by ammonic carbonate (No. XXXIV.), with what occurs when a similar fluid (as in No. XXX.) is contained in a flask sealed during the continuance of ebullition, or also with what occurred in Nos. XIII. and XXXII. In the present case the second boiling seems to have destroyed what small amount of fermentability there was still remaining in the solution; but in No. IX. fermentation did take place after the second boiling--though this occurred only under the influence of diminished pressure and a higher temperature. *No. XXXIV.--Turnip Infusion Neutralized by Ammonic Carbonate* in forty-eight hours showed a slight turbidity, which slowly increased during the next two days. In two days more the turbidity was very great, and there was also a considerable amount of sediment. The fluid was then examined microscopically, and found to contain myriads of large but very languid _Bacteria_. _e. Fluid (in vacuo) in a Flask which had been Sealed during Ebullition._ *No. XXXV.--Healthy Urine* underwent no apparent change for the first twelve days, then (the bulk of the fluid still remaining clear and bright) small greyish white flocculi began to collect at the bottom of the flask, which very slowly increased in quantity during the succeeding twelve days. At the expiration of this time the flocculi were pretty numerous, though the fluid was otherwise bright. The vacuum was ascertained to be still good, and on breaking the flask, the fluid was found to have a slightly acid re-action, though no appreciable odour. When examined microscopically, the flocculi were seen to be made up for the most part of mere granular aggregations (simple, and not in the form of _Bacteria_). Small _Torula_ cells, however, existed in some quantity; also a few necklace-like chains, and a comparatively small number of _Bacteria_, some of which were tolerably active. *No. XXXVI.--Simple Turnip Infusion* after twenty-four hours showed no sign of change, though in thirty-six hours it was slightly turbid. On the fourth day the turbidity was well-marked and general, though there were no flake-like aggregations. When examined microscopically, the fluid was found to contain multitudes of _Bacteria_. *No. XXXVII.--Turnip Infusion,[63] Neutralized by Ammonic Carbonate* in twenty-four hours was decidedly turbid. In thirty-six hours the turbidity was more marked, and there was a slight sediment. By the end of forty-eight hours both turbidity and sediment had notably increased. On the fourth day, there was a moderately clear fluid, containing an abundance of curdy or flake-like masses. When the flask was opened, these were found to be made up principally by the aggregation of myriads of _Bacteria_. [63] Some of same as that which was used (unaltered) in last experiment. FIFTH SET OF EXPERIMENTS (XXXVIII.–XLVII.).