The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
The manner of preparing and mounting many of the Polyzoa and Zoophytes
has been before described; but any notice of _polarizing_ objects would
be incomplete without some allusion to them. A small piece of the
_Flustra avicularis_, well prepared, is beautiful when examined in this
manner. No selenite is needed, and yet the colours are truly gorgeous.
It is often met with upon shells and zoophytes of a large size, and
will well repay the trouble of searching for. Many of the Sertularidæ
are very beautiful with polarized light, and, indeed, no ramble upon
the seaside need be fruitless in this direction.
The different _starches_ are quite a study in themselves, and
are peculiarly connected with polarized light. They are found in
the cellular tissue of almost every plant in small white grains
which vary considerably in size; that from the potato averages
one-three-hundredth of an inch in diameter, and that from arrow-root
about one-six-hundredth. To procure starch from any plant, the texture
must first be broken up or ground coarsely; the mass of matter must be
then well washed in gently-flowing water, and, as all starch is totally
insoluble in cold water, the grains are carried off by the current
and deposited where this is stayed. In procuring it from the potato,
as well as many other vegetables, it is but necessary to reduce the
substance to a coarse pulp by the aid of a culinary “grater;” the pulp
should then be well agitated in water, and allowed to rest a short
time, when the starch will be found at the bottom, its lighter colour
rendering it easily distinguishable from the pulp. It should, however,
be washed through two or three waters to render it perfectly clean.
These grains have no crystalline structure, but present a very peculiar
appearance when examined with polarized light. Each grain shows a dark
cross whose lines meet at the point where it was attached to the plant,
called the _hilum_. Round the grain, also, a series of lines are seen,
as though it were put together in plates. This is more distinctly
visible in some kinds than others.
As to the mounting of these starches there is little to be said. If the
grains are laid upon the slide, and as small a portion as possible of
the balsam diluted with turpentine, as before mentioned, be applied,
they will cling to the glass and allow the pure balsam to flow readily
over them without being so liable to imprison air-bubbles when the thin
glass is put upon them.
The raphides, which were fully described in Chapter II., when required
for use with polarized light, must be mounted in balsam, and many are
found which give beautiful colours. They require no peculiar treatment,
but must be washed quite clean before putting up.
Public-domain text, read in full here on John Shaqi.
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