Ivan's youngest son, Dmitri, with his mother Maria, and her relatives, the Nagois, were domiciled in Uglitch by the order of Boris; whilst there in 1581, about the period of the Tartar invasion, young Dmitri was murdered--at Boris Godunov's instigation it is said. Jerom Horsey, who was in Uglitch at the time, states that he was aroused late at night, the news given him, and his aid requested on behalf of Dmitri's mother believed to be poisoned. Horsey gave the messenger the small vial of sallet oil the Queen (Elizabeth) had given him as a specific against all poisons and ills. An inquiry was ordered when Boris Godunov was suspected of having instigated the crime, and as a result of the investigation made by Shooiski it was declared that the boy cut his own throat and that the Nagois and citizens of Uglitch had put to death innocent men as murderers, whereupon, the incredible finding being believed, an effort was made to exterminate the Nagois, and Uglitch was almost depopulated. There can be no doubt that Dmitri was murdered when six years old, but it is not so clear at whose instigation the deed was done. Giles Fletcher states that the child "resembled his father in delight of blood," and it may be that evidence of his cruel propensities induced some sufferer from Ivan's tyranny to wreak vengeance on the son in hope of saving a generation to come from such suffering as the past had endured. It may be that Boris Godunov plotted for his removal, but it is known that Boris was anxious for Theodore to have a son to succeed to the throne, and, probably, had then little intention of securing it for himself. One of the complaints made by the Russia Company against Jerom Horsey was in connection with a wrongly interpreted order he executed on behalf of Boris Godunov who wished a "wise woman" sent out from England to doctor the Tsaritsa, and the company instead sent out a midwife. To conciliate the small landowners a decree was issued in 1597 forbidding peasants to leave the land and thus serfdom was established. Some efforts had been made in former centuries to restrict the migrations of a people, nomadic by habit, still accustomed to change masters frequently by moving from one estate to another at seed time and harvest. The tendency of the powerful was to increase the size of their holdings and to augment their retainers by enticing labourers from smaller estates. To check this the husbandman was attached to the soil as the serf of the estate.