Francis, afterwards Earl of Bothwell, was the eldest son of John Stewart and Jane Hepburn, sister of that Earl of Bothwell whom Mary Queen of Scots married. Francis succeeded his maternal uncle in title and estates. His father, Lord John Stewart, was an illegitimate son of James V. The Pope, however, legitimized all the natural children of James V; and Mary, after her accession, granted letters of legitimation[150] to her two half-brothers, John Stewart, and James, afterwards the Regent Moray. John was slightly the elder of the two, and had he been legitimate would have been the heir to the exclusion of Mary. The Regent Moray left only daughters, whereas John Stewart had several sons, of whom Francis was the eldest. Francis might therefore claim to be the next heir male to the throne of Scotland, and possibly of England, had James VI died without children. James's own opinion of the matter is shown in his speech to his Parliament in 1592, when he denounced Bothwell as an aspirant to the throne, although he was 'but a bastard, and could claim no title to the crown'. Bothwell, however, was himself no bastard, though his father was. But the significance of the witches' attempt, as well as the identity of the chief personage at their meeting, is given in Barbara Napier's evidence as to the reason for the attempted murder of the King, 'that another might have ruled in his Majesty's place, and the government might have gone to the Devil'. By changing the title 'the Devil' by which he was known to the witches, to the title 'Earl of Bothwell' by which he was known outside the community, the man and the motive are manifest. This hypothesis is borne out by the contemporary accounts. The trial of the witches created a great stir, and Bothwell's name was freely coupled with the witches'. He denied all complicity; this was only natural, as confession would have meant an acknowledgement of high treason. But his followers might have betrayed him. The two leaders, Agnes Sampson and John Fian, were tortured. Sampson admitted that the wax image was made at the instance of Francis, Earl of Bothwell; an admission sufficiently damning, but beyond that she would say nothing. The real danger to Bothwell lay in Fian. Under torture he made admissions and signed a confession in the presence of the King. He was then