Besides 700,264 seeds in stock, or sown. The extreme height attained by the tallest _C. Calisaya_ was, at the same date, fifteen feet, and by the worthless species twenty-eight feet. One of the trees of _C. lancifolia_ had also attained a height of fifteen feet. Dr. de Vry, the eminent chemist who is associated with Dr. Junghuhn, and who had for two years previously occupied himself with the study of the chinchona alkaloids, has been actively engaged in careful investigations of the chinchona barks in Java. With regard to the _C. Calisaya_ his results have been very satisfactory. From the trunk-bark of a plant of this species, six years old, he obtained, in August, 1860, 5 per cent. of alkaloids; and from that of the branches, 2-1/2 per cent. But the specimens of _C. Calisaya_ bark from Java, which have been sent to the Exhibition of 1862, have a very different appearance, and are much thinner than those from South America. This circumstance leads to the inference that the present system of cultivation in Java is erroneous. With the species introduced by M. Hasskarl, Dr. de Vry was not so successful. The leaves, flowers, fruit, and bark of this species were sent to Mr. Howard by Dr. Junghuhn; and it was found that the names of _C. ovata_, given it by M. Hasskarl, and of _C. Lucumæfolia_ by Dr. Junghuhn, were equally erroneous. It was clear that it was one of the numerous worthless species, not previously described, and Mr. Howard, in the seventh number of his work, has named it _C. Pahudiana_,[110] after M. Charles F. Pahud, who, as Minister of the Colonies, sent M. Hasskarl to South America in 1852, and who, being appointed Governor-General of Netherlands India in 1855,[111] did so much to ensure the success of the chinchona experiment in Java. Up to 1860 Dr. de Vry had only obtained 0.4 per cent. of alkaloids from the bark of _C. Pahudiana_, and Mr. Howard's examination coincides with the analysis of Dr. de Vry in pronouncing it an inferior sort. In 1861, however, he obtained 3 per cent. of alkaloids from the bark of the roots of a _C. Pahudiana_ plant eight years old, and 1-1/4 per cent. from the trunk-bark. From a tree aged two years and three months he only got 0.09 per cent. from the trunk-bark, and 1.9 per cent. from the root-bark, of which he states the greater part to be quinine; while in the trunk-bark there was not a trace of that alkaloid. This result leads Dr. de Vry to conjecture that the quinine, once formed in the roots, is employed in the growth of the plant, and that, when it attains its full growth, the trunk-bark will also be rich in quinine. If this should not be the case, he hopes that the roots of the young plants may be used profitably for the manufacture of quinine. It is to be feared that the quinine in the trunk-bark will not increase with age, for, while in the younger tree there was 1.9 per cent. of alkaloids in the roots, chiefly quinine, and 0.09 in the trunk-bark, in the older one there was 3 per cent.