If it be inquired, what reason may be assigned for this? I answer, that it must, in a great measure, be resolved into the sovereignty of God, who will bring his people which way he pleases, to heaven; and may take those comforts which had their first rise from himself; and, at the same time, none must say, why dost thou thus? However, we may observe some particular reasons, which the providence of God points out to us, to which we may in other respects, ascribe our want of assurance; and these may be reduced to four heads, particularly mentioned in this answer. (1.) It is sometimes occasioned by manifold distempers, or bodily diseases: the soul and body are so closely joined to, and dependant on each other, that the one can hardly suffer without the other. Hence it is that bodily distempers affect the mind, excite and give disturbance to the passions; which is a great addition to the uneasiness that ensues hereupon. When the spirits are deprest, and we are under the prevalency of a melancholy disposition, we are oftentimes inclined to think that we are not in a state of grace; and though we were before this disposed to comfort others in like cases, we are at this time unable to take the least encouragement ourselves. All things look black and dismal; our former hope is reckoned no other than delusive, and we brought to the very brink of despair. And it may be observed, that these sad and melancholy apprehensions concerning our state, increase or abate, as the distemper that gives occasion thereunto more or less prevails.