Holmes was put on his trial on October 28, 1895, before the Court of Oyer and Terminer in Philadelphia, charged with the murder of Benjamin Pitezel. In the course of the trial the district attorney offered to put in evidence showing that Holmes had also murdered the three children of Pitezel, contending that such evidence was admissible on the ground that the murders of the children and their father were parts of the same transaction. The judge refused to admit the evidence, though expressing a doubt as to its inadmissibility. The defence did not dispute the identity of the body found in Callowhill Street, but contended that Pitezel had committed suicide. The medical evidence negatived such a theory. The position of the body, its condition when discovered, were entirely inconsistent with self-destruction, and the absence of irritation in the stomach showed that the chloroform found there must have been poured into it after death. In all probability, Holmes had chloroformed Pitezel when he was drunk or asleep. He had taken the chloroform to Callowhill Street as a proposed ingredient in a solution for cleaning clothes, which he and Pitezel were to patent. It was no doubt with the help of the same drug that he had done to death the little children, and failing the nitro-glycerine, with that drug he had intended to put Mrs. Pitezel and her two remaining children out of the way at the house in Burlington; for after his trial there was found there, hidden away in the cellar, a bottle containing eight or ten ounces of chloroform. Though assisted by counsel, Holmes took an active part in his defence. He betrayed no feeling at the sight of Mrs. Pitezel, the greater part of whose family he had destroyed, but the appearance of his third wife as a witness he made an opportunity for "letting loose the fount of emotion," taking care to inform his counsel beforehand that he intended to perform this touching feat. He was convicted and sentenced to death on November 2. Previous to the trial of Holmes the police had made an exhaustive investigation of the mysterious building in Chicago known as "Holmes' Castle." The result was sufficiently sinister. In the stove in the cellar charred human bones were found, and in the middle of the room stood a large dissecting table stained with blood. On digging up the cellar floor some human ribs, sections of vertebrae and teeth were discovered buried in quicklime, and in other parts of the "castle" the police found more charred bones, some metal buttons, a trunk, and a piece of a watch chain. The trunk and piece of watch chain were identified as having belonged to Miss Minnie Williams.