The bitter and implacable hostility borne by the Popes to the German Imperial House of Hohenstauffen led Urban IV. to invite S. Louis, King of France, to assume the title of King of Sicily and Naples. But the delicate conscience of Louis revolted from such an usurpation. If the Crown were hereditary, it belonged to Conradin, grandson of Frederick II., the Great Redbeard, Emperor, King of Germany and of Sicily. But Charles of Anjou, the brother of S. Louis, was less scrupulous. He accepted the invitation. On the death of Urban, Clement IV. pursued the same policy. Manfred, the uncle of Conradin, then wore the Crown of the Sicilies. He was defeated by Charles and fell in battle, 1266, before the army of the Pope and of Charles of Anjou, marching as crusaders. Manfred left an only child, Constance, married to Peter III., King of Aragon. Conradin, at the head of an army, advanced to claim the Crown that was now his by right, regardless of the excommunication and curses hurled at him by the Pope. He was defeated and taken prisoner. Clement, fearful lest Charles should deal leniently towards the last of the Hohenstaufens, wrote to urge him to smother all feelings of pity. “The life of Conradin,” he wrote, “is the death of Charles; the death of Conradin is the life of Charles”; and the Anjou prince had the last male of this noble race executed publicly. As Conradin stood on the scaffold, he flung his glove among the people, crying out that he constituted the King of Aragon his heir. Charles was now King of the Two Sicilies. But he was ambitious of a more splendid title, and he bought that of Jerusalem from Mary of Antioch, daughter of Bohimund V., who inherited the title of King of Jerusalem from his mother, Melusina, daughter of Amaury de Lusignan, twelfth sovereign of the Christian kingdom of Jerusalem. But Jerusalem itself had fallen into the hands of the Saracens in 1244. To return now to Jeanne de Naples. Joanna I. of Naples was born in 1327, and was the daughter of Charles, Duke of Calabria, and of Marie de Valois, his second wife. Charles was the only son of Robert the Good, King of Naples, who was the grandson of Charles of Anjou, brother of S. Louis, to whom had been given the Crown of Naples by Pope Urban IV., determined at any cost to destroy the Hohenstauffen dynasty. Charles, Duke of Calabria, died before his father, and Joanna succeeded to the throne at the age of sixteen.