But just as the tide of opinion was turning strongly in this direction, an utterly unexpected obstacle appeared in the form of the theory of Professor August Weismann, put forward in 1883, which antagonized the Lamarckian conception (though not touching the Darwinian, of which Weismann is a firm upholder) by denying that individual variations, however acquired by the mature organism, are transmissible. The flurry which this denial created has not yet altogether subsided, but subsequent observations seem to show that it was quite disproportionate to the real merits of the case. Notwithstanding Professor Weismann's objections, the balance of evidence appears to favor the view that the Lamarckian factor of acquired variations stands as the complement of the Darwinian factor of natural selection in effecting the transmutation of species. Even though this partial explanation of what Professor Cope calls the "origin of the fittest" be accepted, there still remains one great life problem which the doctrine of evolution does not touch. The origin of species, genera, orders, and classes of beings through endless transmutations is in a sense explained; but what of the first term of this long series? Whence came that primordial organism whose transmuted descendants make up the existing faunas and floras of the globe? There was a time, soon after the doctrine of evolution gained a hearing, when the answer to that question seemed to some scientists of authority to have been given by experiment. Recurring to a former belief, and repeating some earlier experiments, the director of the Museum of Natural History at Rouen, M. F. A. Pouchet, reached the conclusion that organic beings are spontaneously generated about us constantly, in the familiar processes of putrefaction, which were known to be due to the agency of microscopic bacteria. But in 1862 Louis Pasteur proved that this seeming spontaneous generation is in reality due to the existence of germs in the air. Notwithstanding the conclusiveness of these experiments, the claims of Pouchet were revived in England ten years later by Professor Bastian; but then the experiments of John Tyndall, fully corroborating the results of Pasteur, gave a final quietus to the claim of "spontaneous generation" as hitherto formulated.