Margaret, Duchess of Parma, the nominal Regent of the Netherlands, had found her position intolerable since the arrival of the Duke of Alva, and was permitted by Philip to resign (Oct. 6th, 1567). Alva henceforth was untrammelled by even nominal restraint. A process was begun against the Counts Egmont and Hoorn, and William of Orange was proclaimed an outlaw (Jan. 24th, 1568) unless he submitted himself for trial before the _Council of Tumults_. Some days afterwards, his eldest son, a boy of fifteen and a student in the University of Louvain, was kidnapped and carried off to Spain.[262] William replied in his famous _Justification of the Prince of Orange against his Calumniators_, in which he declared that he, a citizen of Brabant, a Knight of the Golden Fleece, a Prince of the Holy Roman Empire, one of the sovereign Princes of Europe (in virtue of the principality of Orange), could not be summoned before an incompetent tribunal. He reviewed the events in the Netherlands since the accession of Philip II., and spoke plainly against the misgovernment caused, he said diplomatically, by the evil counsels of the King's advisers. The _Justification_ was published in several languages, and was not merely an act of defiance to Philip, but a plea made on behalf of his country to the whole of civilised Europe. The earlier months of 1568 had been spent by the Prince of Orange in military preparations for the relief of his countrymen, and in the spring his army was ready. The campaign was a failure. Hoogstraten was defeated. Louis of Nassau had a temporary success at Heiliger-Lee (May 23rd, 1568), only to be routed at Jemmingen (July 21st, 1568). After William had issued a pathetic but unavailing manifesto to Protestant Europe, a second expedition was sent forth only to meet defeat. The cause of the Netherlands seemed hopeless. But Alva was beginning to find himself in difficulties. On the news of the repulse of his troops at Heiliger-Lee he had hastily beheaded the Counts Egmont and Hoorn. Instead of striking terror into the hearts of the Netherlanders, the execution roused them to an undying hatred of the Spaniard. He was now troubled by lack of money to pay his troops. He had promised Philip to make gold flow from the Low Countries to Spain; but his rule had destroyed the commerce and manufactures of the country, the source of its wealth. He was almost dependent on subsidies from Spain. Elizabeth of England had been assisting her fellow Protestants in the way she liked best, by seizing Spanish treasure ships; and Alva was reduced to find the money he needed within the Netherlands.