Prof. Mallet concludes that "there are no sound grounds on which to establish such general standards of purity as have been proposed, looking to exact amounts of organic carbon or nitrogen, albuminoid ammonia, oxygen of permanganate consumed, etc., as permissible or not. Distinctions drawn by the application of such standards are arbitrary and may be misleading." While this is perfectly true, considered from the standpoint of scientific precision, it does not sufficiently take into account the value of probabilities in these matters, considered as motives to action. It is perfectly true that there can be no fixed standard--that a water which the chemist would report as relatively pure might be much more apt to produce disease than one which he would pronounce impure--but it is nevertheless true that from the results of chemical analysis, taken in connection with evidence as to the source and history of the water, an opinion can be formed as to the danger from its use which is sufficiently reliable to be acted upon in the absence of positive evidence, such as the production of disease. In many cases the matter must be doubtful, and Prof. Mallet truly says that it will not do in all such cases to forbid the use of the water, for it often happens that this should not be done unless it is absolutely necessary; but there are many other cases in which there is very little doubt, and where action should be governed by the probabilities. The microscopical examination of suspected waters sometimes gives decided indication as to the nature of the impurities; and it may be that hereafter, in connection with physiological tests, it will become of even more importance than the chemical. To determine the presence of organisms in a sample of water the best method known at present is to kill and coagulate them by means of osmic acid or chloride of platinum, and allow them to subside. This method is of course inapplicable if it be desired to use them for either culture- or inoculation-tests. Chemists have no uniform system of reporting the results of their analyses, some using grains per gallon, U.S. or Imperial as may be, and others parts per hundred thousand or per million of the water. It is therefore difficult to appreciate the value of the figures as given by them. The following, in parts per 100,000, will enable the practitioner to form a general estimate of the character of analytical reports; but the opinion in individual cases is so modified by the coincident amounts of chlorine, ammonia, nitrous and nitric acids, that the experienced sanitarian only is qualified to put on the results an estimate which shall be in accordance with our present knowledge of such matter: _Upland Surface-Waters_. Allowable. Doubtful. Impure. Total organic elements to .4 .4 to .6 Over .6 Oxygen required to .3 .3 to .4 " .4 Albuminoid ammonia to .015 .015 to .025 " .025