───────────────────────┬───────────────┬───────┬────────── Time after Addition │ C.c. of CO{2} │ Time. │ C.c. per of Toluene, Minutes │ per Minute. │ │ Minute ───────────────────────┴───────────────┴───────┴────────── 0 4·6 6 1·6 1 4 8 1·2 2 3·3 12 0·85 3 2·6 24 0·8 4 2 32 0·5 5 1·8 constant ────────────────────────────────────────────────────────── Simultaneously with this, the yeast acquires the property of decomposing and fermenting hexosephosphate and of responding to the addition of phosphate. This last property is only acquired to a small degree in this way but it becomes much more strongly developed if the pressed yeast be washed with toluene on the filter pump. Thus 10 grams of yeast after this treatment fermented fructose at 1·2 c.c. per three minutes; after the addition of phosphate (5 c.c. of 0·6 molar phosphate) the rate rose to 6·9 and then gradually fell in the typical manner [Harden, 1910; see also Euler and Johansson, 1912, 3]. The current explanation of the great decrease in rate of fermentation which attends the action of toluene and other antiseptics on living yeast, and also follows upon the disintegration of the cell, appears to be that in living yeast the high rate of fermentation is maintained by the continued production of relatively large fresh supplies of fermenting complex, and that when the power of producing this catalytic agent is destroyed by the poison, the rate of fermentation falls to a low value, corresponding to the store of zymase still present in the cell (cf. Buchner, E. and H., and Hahn, 1903, pp. 176, 180).