An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.Griffith, J. W. (John William)
Science
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.
Griffith, J. W. (John William)
Microscopy
In the best object-glasses the aberrations are so well balanced that the
mere covering an object with thin glass is sufficient to disturb the
balance and render very delicate markings either misty and coloured or
wholly invisible. The effect produced by a plate of glass may be
understood by reference to fig. 26, the rays being supposed to emanate
from the object at _a_; and it is evident that the refraction of the
glass so alters the direction of the rays that they will fall upon the
lower combination nearer the centre than if the cover were absent, and
thus negative aberration is produced. In the best object-glasses,
however, this aberration may almost entirely be removed, the lower
combination being susceptible of approximation by a screw movement to
the second or next above it, so that the ascending rays, being able to
continue their oblique course through the increased distance between the
object and the lower combination, may fall upon the same portions of the
latter that they did before the cover was applied.
POLARIZATION OF LIGHT.--In attempting to give a sketch of this curious
and difficult subject, we must suppose the reader to be in possession of
a natural crystal of calcareous spar, and either two Nicol’s prisms
(forming the ordinary polariscope) or two plates of the mineral called
tourmaline cut in the direction of the length or axis of the crystal.
Hitherto we have considered rays of light falling upon transparent
substances as simply refracted or reflected according to the ordinary
laws of refraction or reflexion. We have now to notice some curious
exceptions, forming the basis of many interesting phenomena, especially
in connexion with the microscope, in which these laws are more or less
deviated from. If we place a plate of tourmaline, cut as above directed,
upon or beneath the stage of the microscope, the light will pass through
it, appearing tinged with the green or brown colour natural to the
tourmaline; but on laying another slice upon the eye-piece, and turning
the latter round or rotating it, the light will be transmitted in
certain positions only, being partially or entirely arrested in others,
so that the field appears black. And, on careful examination, it will be
noticed that the change from black to white occurs at each quarter of a
rotation, being twice black and twice white in an entire rotation, the
changes occurring alternately. The same phenomena may also be exhibited
by substituting two Nicol’s prisms for the tourmalines.
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