An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.Griffith, J. W. (John William)
Science
An Elementary Text-book of the Microscope: including a description of the methods of preparing and mounting objects, etc.
Griffith, J. W. (John William)
Microscopy
In fig. 30 the analyzer is represented as composed of a natural crystal
(rhomb) of calcareous spar, which transmits both sets of rays; but in
the ordinary analyzer or Nicol’s prism--which is made by dividing a
rhomb through the obtuse angles into two wedge-shaped pieces and
cementing them together again with balsam, only one set of rays is
transmitted at each quarter-revolution, the other being refracted out of
the field. In the case of the tourmaline, one of the sets of rays is
absorbed; so that the tourmaline, like the Nicol’s prism, is
single-imaged.
Thus the colours produced by polarization are the same as those of the
spectrum, but separated in a different way, both arising from the
elementary coloured rays of the compound white light. For while the
spectral rays are separated by dispersive refraction, the polarized
coloured rays are separated by the interference and annihilation of some
rays, the remainder passing on to produce the colours.
When the position of the depolarizing crystal is such that the plane of
the polarized light coincides with the direction of the optic axis or
axes, the light is not doubly refracted nor polarized in certain parts;
hence these parts appear white if the plane of the polarizer and
analyzer coincide, and black if they be crossed. A crystal cut at right
angles to its optic axis, with its length directed towards the polarizer
and analyzer, is in this position, and it exhibits alternately a black
and white cross, with one or two sets of concentric rings of
complementary colours at each quarter of a rotation.
To prepare crystals for examination by polarized light, a little Epsom
salt, nitre, or borax should be dissolved in water, a drop placed upon a
slide, and dried at a gentle heat. The crystals should then be mounted
in balsam, and viewed as transparent objects.
To see the cross and rings, the crystals should be sawn or cut across
transversely, the ends being polished on a strained piece of silk
moistened with water, and the sections mounted in balsam.
The property of doubly refracting and polarizing light is not confined
to crystalline substances, being also possessed by many organic bodies,
for the details of which I must refer to the article Polarization in the
‘Micrographic Dictionary.’
INDEX.
Abdomen, 129.
Aberration, chromatic, 173.
Aberration, spherical, 172.
Acalephæ, 153.
Acarus, 131.
Accumbent, 44.
Achromatic, 178.
Achromatic condenser, 5.
Achromatism, 177.
Acicular, 24.
Acotyledons, 43.
Acrocarpi, 55.
Acrostalagmus, 111.
Actinocyclus, 80.
Actinophrys, 156.
Adapter, 2.
Adulteration, 23.
Æcidium, 100.
Agaricus, 97.
Albumen, of seeds, 43.
----, of eggs, 113.
Algæ, 64.
Alyscum, 161.
Amœba, 155.
Amphileptus, 162.
Angiocarpi, 94.
Angle of vision, 175.
Anguillula, 152.
Angular, 23.
Animal tissues, 113.
Ankistrodesmus, 74.
Annular vessels, 27.
Annulus, of Ferns, 50.
----, of Fungi, 98.
----, of Mosses, 55.
Anoplura, 135.
Ant, 143.
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