Herr Kleine, in his pamphlet on Italian Bees and Bee-keeping (Berlin, 1855), says that on substituting during the absence of the bees a hive filled with empty comb for their own hive, the returning bees exhibit the utmost perplexity. As the substituted hive stands in the exact spot previously occupied by their own hive, the returning bees fly into it without observing the change. But finding only empty combs inside, 'they stop, do not know where they are, come out of the hole again without depositing their loads, fly off, look most carefully round the stand to assure themselves that they have made no mistake, and go in once more when convinced that they are at the right place. The same thing is repeated over and over again, until the bees at last bow to the incomprehensible and unavoidable, lay down their loads, and set to work at those tasks made necessary by the new circumstances of the hive. But as all the newly arriving bees behave in similar fashion, the disturbance lasts till late in the evening, and the uncertainty and anxiety of the bees is so great that the bee-master cannot contemplate it without deep sympathy.' Under such circumstances the bees take quickly to a substituted queen; 'for the feeling of the first comers that they have no right to the new dwelling, having, as they suppose, made some inexplicable mistake which they cannot remedy, prevents them from feeling any hostility to the new queen which they find; they probably consider themselves as merely on sufferance, and feel that they should be grateful that no action is taken against them for their illegal entry, as generally happens in bee-experience.' Hence the writer adopts this device when he desires to exchange or substitute queens. Büchner, after alluding to this case, supplements it with the following:-- The wind threw down from the stand of a bee-master--a friend of the author's, whose name will soon become known--a straw beehive, the inmates of which were surprised in full work, and no small disorder in the interior was the result. The owner repaired the hive, put the loose comb back in its place, and replaced it in such a manner that the wind could not again catch it, hoping that the accident would have no further results. But when he examined the hive a few days later, he found that the bees had left their old home in the lurch, and had tried to enter other hives, clearly because they could no longer trust the weather, and feared that the terrible accident might again befall them.