It remains yet in the order of logic, though not of history, to show that among these solid destructible particles there really do exist germs capable of giving rise to the development of living forms in suitable menstrua. This piece of work was done by M. Pasteur in those beautiful researches which will ever render his name famous; and which, in spite of all attacks upon them, appear to me now, as they did seven years ago, to be models of accurate experimentation and logical reasoning. He strained air through cotton-wool, and found, as Schroeder and Dusch had done, that it contained nothing competent to give rise to the development of life in fluids highly fitted for that purpose. But the important further links in the chain of evidence added by Pasteur are three. In the first place he subjected to microscopic examination the cottonwool which had served as strainer, and found that sundry bodies clearly recognisable as germs were among the solid particles strained off. Secondly, he proved that these germs were competent to give rise to living forms by simply sowing them in a solution fitted for their development. And, thirdly, he showed that the incapacity of air strained through cotton-wool to give rise to life was not due to any occult change effected in the constituents of the air by the wool, by proving that the cotton-wool might be dispensed with altogether, and perfectly free access left between the exterior air and that in the experimental flask. If the neck of the flask is drawn out into a tube and bent downwards; and if, after the contained fluid has been carefully boiled, the tube is heated sufficiently to destroy any germs which may be present in the air which enters as the fluid cools, the apparatus may be left to itself for any time and no life will appear in the fluid. The reason is plain. Although there is free communication between the atmosphere laden with germs and the germless air in the flask, contact between the two takes place only in the tube; and as the germs cannot fall upwards, and there are no currents, they never reach the interior of the flask. But if the tube be broken short off where it proceeds from the flask, and free access be thus given to germs falling vertically out of the air, the fluid, which has remained clear and desert for months, becomes, in a few days, turbid and full of life. CCXXI