1886 2,671 25 0.94 1887 1,770 14 0.79 1888 1,622 9 0.55 1889 1,830 7 0.38 1890 1,540 5 0.32 1891 1,559 4 0.25 1892 1,790 4 0.22 1893 1,648 6 0.36 1894 1,387 7 0.50 1895 1,520 2 0.13 1896 1,308 4 0.30 1897 1,521 6 0.39 Pasteur's treatment of rabies by inoculation of emulsions of dried spinal cord is, therefore, a "vaccination" of attenuated virus, resulting in antitoxin formation, to the further protection of the individual against rabies. One further example of the modern application of the principles of active acquired immunity may be shortly mentioned. We refer to the cholera and plague vaccinations. The vaccination in small-pox is an inoculation of the _virus of the disease_; the rabies inoculation is a transmission of the _vital products of the disease attenuated_; the plague and cholera vaccinations are inoculations of _pure cultures of living virus_ from outside the body. Inoculating cholera virus against cholera has been made illegal, as variolation was in 1840. But Haffkine has prepared two vaccines. The weak one is made from pure cultures of Koch's spirillum of Asiatic cholera, attenuated by growth to several generations on agar or broth at 39°C. The strong one is from similar culture the virulence of which has been increased. One cubic centimetre of the first vaccine is injected hypodermically into the flank, and the second vaccine three or four days afterwards. The immunisation is prophylactic, not remedial, and its action takes effect five or six days after the second vaccine has been injected. INOCULATION TREATMENT FOR PERSONS AFFECTED WITH RABIES