The negative results in looking for active alkaloidal, or glucosidal, or proteid bodies suggested that perhaps the action was due to some inorganic constituent. The writer then boiled the extract three minutes and as the filtrate was still found active and the proteid precipitate inactive became convinced of the inorganic nature of the active constituents, and finally incinerated the plant. The acid extract from this was also active, but death was delayed several hours. This was believed to be due to the insoluble form into which the compound was converted.[158] In fact, the question of solubility and the avoidance of an acid reaction, which of itself may kill, are the main points to keep in mind. These experiments indicated that the injurious action toward rabbits of the _Astragalus mollissimus_ and _Aragallus lamberti_ collected at Hugo, Colo., was due to one or more inorganic constituents,[159] but it does not follow that all loco plants have the same poisonous principle nor that the same species occurring on all soils has the same poisonous action.[160] Of _Astragalus mollissimus_ from Imperial, Nebr., collected in 1906, 200 grams were ashed in a platinum bowl and extracted with water. This aqueous extract when neutralized produced no marked symptoms in a rabbit and the weight of the animal remained about the same. The ash undissolved after this extraction was then treated with acetic acid and water overnight, and after carefully evaporating off the acetic acid on the bath (tested by litmus paper) the residue was fed, partly in solution and partly suspended in water, to a rabbit weighing 1,800.2 grams. Next day the rabbit weighed 1,771.8 grams, showed paralysis of the limbs, and died during the morning. The stomach was intensely reddened and contracted. An extract of a similar ash was made by boiling the same amount with a large quantity of 94 per cent alcohol. This was evaporated in vacuo and taken in water and fed to a rabbit weighing 1,459.9 grams. On the sixth day the animal died, having lost 70.9 grams in weight. The stomach showed reddening but no ulcers. An acetic acid aqueous extract, made from the ash after the alcoholic extraction, proved inactive, showing that the alcohol had removed the active bodies. A 70 per cent alcohol extract of another ashed lot proved active, killing the rabbit overnight. Of _Astragalus mollissimus_ from Imperial, Nebr., 200 grams were ashed in a platinum bowl and the ash treated with acetic acid water. After freeing from acid, one half of the solution and emulsion was fed one day and the second half fed the following day. The rabbit at the time of feeding weighed 1,275.7 grams. Fourteen days later the animal died, weighing 1,105.6 grams. No autopsy. A similar extract of the ash from between 100 and 150 grams of the same dried plant produced death in a rabbit weighing 1,190 grams in two hours and fifty-eight minutes.