One lot of _Aragallus lamberti_ collected at Hugo, Colo., in May, 1905, and which gave an ash content of 37.3 per cent, was found to yield 3 milligrams of BaSO_{4} from 1.998 grams of ash, or 173.88 milligrams of Ba(C_{2}H_{3}O_{2})_{2}+H_{2}O in 200 grams of the dried plant, but this ash also contained 0.27 per cent of SO_{3}. The Bureau of Chemistry reported the barium to correspond to 2.9 milligrams of BaSO_{4} in 2.45 grams of the ash. The _Astragalus missouriensis_ (Hugo, June, 1907), with an ash content of 21.8 per cent, gave 3 milligrams of BaSO_{4} in 2.01 grams of ash, or 76.58 milligrams of Ba(C_{2}H_{3}O_{2})_{2}+H_{2}O in 200 grams of the dried plant. The residue after weighing was tested spectroscopically and gave a bright barium spectrum. The _Astragalus drummondii_ from Custer, S. Dak. (1906), _Astragalus mollissimus_ from Kit Carson County, Colo. (December, 1906), and _Astragalus nitidus_ from Woodland Park, Colo. (October, 1907), were reported by the Bureau of Chemistry to contain no barium. The ash of the _Astragalus pectinatus_ (Hugo, June, 1907) was reported by the Bureau of Chemistry to show no barium on spectroscopic examination. Two grams of active loco plant ash yielded from 5 to 6 milligrams of BaSO_{4}, but it can be easily seen that in multiplying this amount to correspond to 200 grams of the dried plant errors would be likely to arise, so that the whole amount of barium would not necessarily be accounted for. * * * * * FOOTNOTES: [188] Hillebrand, W. F. Analysis of Silicate and Carbonate Rocks. U. S. Geol. Surv. Bul. 305, p. 116. 1907. See also Folin, O., On the Reduction of Barium Sulphate in Ordinary Gravimetric Determinations, in Journ. Biol. Chem., vol. 3, p. 81. 1907. [189] All the determinations of barium which resulted either positively or negatively were made with the same bottle of sodium carbonate and H_{2}SO_{4}, so that impurities in the chemicals were thus eliminated. [190] Report from Bureau of Chemistry. =ANALYSIS OF SOILS.= One sample of the soil from near Hugo, Colo., from which the _Aragallus lamberti_ was collected, was examined by the Bureau of Soils, and that Bureau reported the absence of barium and zirconium, at least of any recognizable by the chemical methods used, so that it can not be said that the barium came from any soil accidentally mixed with the ash. Traces of titanium were, however, found. Evidently the plant must collect minimal quantities of these elements from the soil and store them. The water from a well of an adjacent area was examined by the Bureau of Chemistry and reported to contain 37.4 parts of calcium and 13.7 parts of magnesium in one million, and that the water contained no barium.[191] * * * * * FOOTNOTES: