As the master and scholar were near relations, Mr. Guthrie was his peculiar care, and lodged, when at the college, in the same chamber with him, and therefore had the principles of learning infused into him with more accuracy than his class-fellows. Having taken the degree of master of arts, he applied himself, for some years, to the study of divinity, under the direction of Mr. Samuel Rutherford. Mr. Trail says, "Then and there it pleased the Lord, who separated him from his mother's womb, to call him, by his grace, by the ministry of excellent Mr. Samuel Rutherford, and this young gentleman became one of the first fruits of his ministry at St. Andrews. His conversion was begun with great terror of God in his soul, and completed with that joy and peace in believing that accompanied him through his life. After this blessed change wrought upon him, he resolved to obey the call of God to serve him in the ministry of his gospel, which was given him by the Lord's calling him effectually to grace and glory. He did for this end so dispose of his outward estate, (to which he was born heir) as not to be intangled with the affairs of this life." He gave his estate to the only brother of the five who was not engaged in the sacred office, that thereby he might be perfectly disintangled from the affairs of this life, and entirely employed in these of the eternal world. Soon after he was licensed to preach, he left St. Andrews, with high esteem and approbation from the professors of that university, which they gave proof of, by their ample recommendations. After this he became tutor to lord Mauchlin, eldest son to the earl of Loudon; in which situation he continued for some time, till he entered upon a parochial charge. The parish of Kilmarnock, in the shire of Ayr, being large, and many of the people, belonging to the said parish, being no less than six or seven miles distant from their own kirk; for which and other reasons the heritors and others procured a disjunction, and called the new parish Fen wick or new Kilmarnock. Mr. Guthrie was employed to preach at Galston, on a preparation-day before the celebration of the Lord's supper; and several members of the new-erected parish, were present at that occasion, who, being greatly edified by his sermons, conceived such a value for him, that they immediately resolved to make choice of him for their minister; and in consequence thereof gave him a very harmonious call, which he complied with. It is said, that he, along with the people, made choice of the place of ground for building the church upon, and preached within the walls of the house before it was completed; which bears the date of being built _anno_ 1643; and he was ordained unto the sacred office Nov. 7, 1644.