Two Directions of Growth.--Physiology, established on the broad foundations of Müller, developed along two independent pathways, the physical and the chemical. We find a group of physiologists, among whom Weber, Ludwig, Du Bois-Reymond, and Helmholtz were noteworthy leaders, devoted to the investigations of physiological facts through the application of measurements and records made by machinery. With these men came into use the time-markers, the myographs, and the ingenious methods of recording blood-pressure, changes in respiration, the responses of muscle and nerve to various forms of stimulation, the rate of transmission of nerve-currents, etc. The investigation of vital activities by means of measurements and instrumental records has come to represent one especial phase of modern physiology. As might have been predicted, the discoveries and extensions of knowledge resulting from this kind of experimentation have been remarkable, since it is obvious that permanent records made by mechanical devices will rule out many errors; and, moreover, they afford an opportunity to study at leisure phenomena that occupy a very brief time. The other marked line of physiological investigation has been in the domain of chemistry, where Wöhler, Liebig, Kühne, and others have, through the study of the chemical changes occurring in its body, observed the various activities that take place within the organism. They have reduced all tissues and all parts of the body to chemical analysis, studied the chemical changes in digestion, in respiration, etc. The more recent observers have also made a particular feature of the study of the chemical changes going on within the living matter.