Spallanzani thought that the experiments of Needham had not been conducted with sufficient care and precision; accordingly, he made use of glass flasks with slender necks which could be hermetically sealed after the nutrient fluids had been introduced. The vials which Needham used as containers were simply corked and sealed with mastic, and it was by no means certain that the entrance of air after heating had been prevented; moreover, no record was made by Needham of the temperature and the time of heating to which his bottles and fluids had been subjected. [Illustration: Fig. 90.--Lazzaro Spallanzani, 1729-1799.] Spallanzani took nutrient fluids, such as the juices of vegetables and meats which had been extracted by boiling, placed them in clear flasks, the necks of which were hermetically sealed in flame, and afterward immersed them in boiling water for three-quarters of an hour, in order to destroy all germs that might be contained in them. The organic infusions of Spallanzani remained free from change. It was then, as now, a well-known fact that organic fluids, when exposed to air, quickly decompose and acquire a bad smell; they soon become turbid, and in a little time a scum is formed upon their surface. The fluids in the flasks of Spallanzani remained of the same appearance and consistency as when they were first introduced into the vessel, and the obvious conclusion was drawn that microscopic life is not spontaneously formed within nutrient fluids. "But Needham was not satisfied with these results, and with a show of reason maintained that such a prolonged boiling would destroy not only germs, but the germinative, or, as he called it, the 'vegetative force' of the infusion itself. Spallanzani easily disposed of this objection by showing that when the infusions were again exposed to the air, no matter how severe or prolonged the boiling to which they had been subjected, the infusoria reappeared. His experiments were made in great numbers, with different infusions, and were conducted with the utmost care and precision" (Dunster). It must be confessed, however, that the success of his experiments was owing largely to the purity of the air in which he worked, the more resistant atmospheric germs were not present: as Wyman showed, long afterward, that germs may retain their vitality after being subjected for several hours to the temperature of boiling water.