He devised a box, or chamber, as shown in Fig. 91, having in front a large glass window, two small glass windows on the ends, and in the back a little air-tight trap-door. Through the bottom of this box he had fitted ordinary test tubes of the chemist, with an air-tight surrounding, and on the top he had inserted some coiled glass tubes, which were open at both ends and allowed the passage of air in and out of the box through the tortuous passage. In the middle of the top of the box was a round piece of rubber. When he perforated this with a pinhole the elasticity of the rubber would close the hole again, but it would also admit of the passage through it of a small glass tube, such as is called by chemists a "thistle tube." The interior of this box was painted with a sticky substance like glycerin, in order to retain the floating particles of the air when they had once settled upon its sides and bottom. The apparatus having been prepared in this way, was allowed to stand, and the floating particles settled by their own weight upon the bottom and sides of the box, so that day by day the number of floating particles became reduced, and finally all of them came to rest. The air now differed from the outside air in having been purified of all of its floating particles. In order to test the complete disappearance of all particles. Tyndall threw a beam of light into the air chamber. He kept his eye in the darkness for some time in order to increase its sensitiveness; then, looking from the front through the glass into the box, he was able to see any particles that might be floating there. The floating particles would be brightly illuminated by the condensed light that he directed into the chamber, and would become visible. When there was complete darkness within the chamber, the course of the beam of light was apparent in the room as it came up to the box and as it left the box, being seen on account of the reflection from the floating particles in the air, but it could not be seen at all within the box. When this condition was reached, Tyndall had what he called optically pure air, and he was now ready to introduce the nutrient fluids into his test tubes. Through a thistle tube, thrust into the rubber diaphragm above, he was able to bring the mouth of the tube successively over the different test tubes, and, by pouring different kinds of fluids from above, he was able to introduce these into different test tubes. These fluids consisted of mutton broth, of turnip-broth, and other decoctions of animal and vegetable matter. It is to be noted that the test tubes were not corked and consequently that the fluids contained within them were freely exposed to the optically pure air within the chamber.