A series of complement fixation tests on blood specimens from 466 of the inmates show, however, that a very much larger number are infected with either syphilis or gonorrhoea or both of these diseases.[307] With the Wassermann test 176, or 37.7 percent gave positive reactions; 273, or 58.6 percent gave negative reactions, and 17, or 3.6 percent gave doubtful reactions. With a modification of the Wassermann technique where the tests were allowed to stand for four hours at ice box temperature to fix complement, instead of the usual one hour at 37°C. in the incubator, 224, or 48 percent gave positive reactions, 212, or 45.4 percent gave negative reactions and 30, or 6.4 percent gave doubtful reactions, showing an increase of 10.3 percent of positive reactions for syphilis over the method of fixing complement at 37°C. The same sera were tested by the complement fixation test for gonorrheal infection with the result that 134 or 29 percent gave positive reactions; 234, or 50 percent gave negative reactions and 98, or 21 percent gave doubtful reactions, fixing complement at 37°C. for one hour. When the ice box method of fixation was used, 306 or 65.6 percent gave positive reactions; 101, or 21.7 percent gave negative reactions and 59, or 12.6 percent gave doubtful reactions, showing an increase of 36.9 percent of positive results over the method of fixing complement at 37°C. in the incubator. Vaginal smears from the same persons were examined but it was possible to demonstrate the presence of the gonococcus in but five of them, although many of them show the presence of numerous pus corpuscles.[308] The full significance of the results above stated does not appear until the statistics are summarized. Of the 466 girls tested, only 50, that is, 10.7 percent, are found to be free from venereal infection. Practically 90 percent showed infection; 170, or 36.4 percent gave positive reactions for both syphilis and gonorrhoea; 27, or 5.79 percent were positive for syphilis only, and 117, or 25.1 percent were positive for gonorrhoea only.[309]