It seemed necessary in these experiments to imitate as completely as possible by chemical agencies the effects of the spermatozoon upon the egg. When a spermatozoon enters the egg of a sea-urchin or certain starfish or annelids, the immediate effect is a characteristic change of the surface of the egg, namely the formation of the so-called membrane of fertilisation. The writer found that we can produce this membrane in the unfertilised egg by certain acids, especially the monobasic acids of the fatty series, e.g. formic, acetic, propionic, butyric, etc. Carbon-dioxide is also very efficient in this direction. It was also found that the higher acids are more efficient than the lower ones, and it is possible that the spermatozoon induces membrane-formation by carrying into the egg a higher fatty acid, namely oleic acid or one of its salts or esters. The physico-chemical process which underlies the formation of the membrane seems to be the cause of the development of the egg. In all cases in which the unfertilised egg has been treated in such a way as to cause it to form a membrane it begins to develop. For the eggs of certain animals membrane-formation is all that is required to induce a complete development of the unfertilised egg, e.g. in the starfish and certain annelids. For the eggs of other animals a second treatment is necessary, presumably to overcome some of the injurious effects of acid treatment. Thus the unfertilised eggs of the sea-urchin Strongylocentrotus purpuratus of the Californian coast begin to develop when membrane-formation has been induced by treatment with a fatty acid, e.g. butyric acid; but the development soon ceases and the eggs perish in the early stages of segmentation, or after the first nuclear division. But if we treat the same eggs, after membrane-formation, for from 35 to 55 minutes (at 15 deg C.) with sea-water the concentration (osmotic pressure) of which has been raised through the addition of a definite amount of some salt or sugar, the eggs will segment and develop normally, when transferred back to normal sea-water. If care is taken, practically all the eggs can be caused to develop into plutei, the majority of which may be perfectly normal and may live as long as larvae produced from eggs fertilised with sperm.