Directions for Collecting and Preserving InsectsRiley, Charles V. (Charles Valentine)
Science
Directions for Collecting and Preserving Insects
Riley, Charles V. (Charles Valentine)
Insects -- Collection and preservation
_Inflation of the Larvae of Lepidoptera._--The larvae of Lepidoptera
preserved in alcohol are excellent for anatomical and general study, but
are not very suitable for use in economic displays. This means of
preservation also has the disadvantage of not generally preserving the
natural color and appearance of the specimens. These objections may be
avoided, however, by the dry method of preserving larvae, viz, by blowing
or inflation. The process may be described as follows: The larva may be
operated upon alive, but should preferably be first killed by dipping in
chloroform or alcohol, or in the cyanide bottle. It is then placed on a
piece of blotting paper and the alimentary canal caused to protrude from
one-eighth to one-fourth of an inch, by rolling a pencil over the larva
from the head to the posterior extremity. The protruding tip is then
severed with a sharp knife or pair of dissecting scissors, and the
contents of the abdomen are forced out by passing a pencil, as before, a
number of times over the larva. Great care should be exercised in
expressing the fluids not to press the pencil too strongly against the
larva or to continue the operation too long, as this will, especially in
delicate larvae, remove the pigment from the skin, and the specimen when
dried will show discolored spots and be more or less distorted. The
larva should be moved from place to place on the blotting paper during
the operation, so as not to become soiled by its own juices. A straw, or
a glass tube drawn to a point at the tip, is then inserted in the
protruding portion of the alimentary canal. If a straw is used the larva
may be fastened to it by thrusting a pin through the wall of the canal
and the straw. In the case of the glass tube the alimentary canal can be
caused to adhere by drying for a few minutes and this operation may be
hastened and the fastening made more secure by touching the point of
union with a drop of glue. The straw or glass tube is then attached to a
small rubber bag, previously inflated with air, the ordinary dentist's
or chemist's gas bag answering admirably for this purpose. The larva is
now ready for drying, and for this purpose a drying oven is required
into which it is thrust and manipulated by turning it from side to side,
to keep it in proper shape and dry it uniformly until the moisture has
been thoroughly expelled. An apparatus which I have found very
convenient for this purpose is represented at Fig. 109. It consists of a
tin box with mica or glass slides, _e_, to allow the larva to be
constantly in sight. It has also a hinged top, _b_, which may be kept
closed or partly open, or entirely open, as may be necessary, during the
operation. The ends of the box are prolonged downward about 5 inches,
forming supports for it, _g_. Beneath it is placed an alcohol lamp, _f_,
which furnishes the heat. In the end of the box is a circular opening,
_d_, for the introduction of the larva, and this may be entirely or
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