Schulze and Schwann took up the question from this point of view in 1836 and 1837. The passage of air through red-hot glass tubes, or through strong sulphuric acid, does not alter the proportion of its oxygen, while it must needs arrest, or destroy, any organic matter which may be contained in the air. These experimenters, therefore, contrived arrangements by which the only air which should come into contact with a boiled infusion should be such as had either passed through red-hot tubes or through strong sulphuric acid. The result which they obtained was that an infusion so treated developed no living things, while, if the same infusion was afterwards exposed to the air, such things appeared rapidly and abundantly. The accuracy of these experiments has been alternately denied and affirmed. Supposing then, to be accepted, however, all that they really proved was that the treatment to which the air was subjected destroyed _something_ that was essential to the development of life in the infusion. This "something" might be gaseous, fluid, or solid; that it consisted of germs remained only an hypothesis of greater or less probability. Contemporaneously with these investigations a remarkable discovery was made by Cagniard de la Tour. He found that common yeast is composed of a vast accumulation of minute plants. The fermentation of must, or of wort, in the fabrication of wine and of beer, is always accompanied by the rapid growth and multiplication of these _Toruloe_. Thus, fermentation, in so far as it was accompanied by the development of microscopical organisms in enormous numbers, became assimilated to the decomposition of an infusion of ordinary animal or vegetable matter; and it was an obvious suggestion that the organisms were, in some way or other, the causes both of fermentation and of putrefaction. The chemists, with Berzelius and Liebig at their head, at first laughed this idea to scorn; but in 1843, a man then very young, who has since performed the unexampled feat of attaining to high eminence alike in Mathematics, Physics, and Physiology-- I speak of the illustrious Helmholtz--reduced the matter to the test of experiment by a method alike elegant and conclusive. Helmholtz separated a putrefying or a fermenting liquid from one which was simply putrescible or fermentable by a membrane which allowed the fluids to pass through and become intermixed, but stopped the passage of solids. The result was, that while the putrescible or the fermentable liquids became impregnated with the results of the putrescence or fermentation which was going on on the other side of the membrane, they neither putrefied (in the ordinary way) nor fermented; nor were any of the organisms which abounded in the fermenting or putrefying liquid generated in them. Therefore the cause of the development of these organisms must lie in something which cannot pass through membranes; and as Helmholtz's investigations were long