=230. Anaerobic respiration.=—The last experiment is also an excellent one to show _anaerobic_ respiration. In the tube filled with mercury so that when inverted there will be no air, it will be seen after 24 hours that a gas has accumulated in the tube which has crowded out some of the mercury. With a wash bottle which has an exit tube properly curved, some water may be introduced in the tube. Then insert underneath a small stick of caustic potash. This will form a solution of potash, and the gas will be partly or completely absorbed. This shows that the gas was carbon dioxide. This evolution of carbon dioxide by living plants when there is no access of oxygen is anaerobic respiration (sometimes called intramolecular respiration). It occurs markedly in oily seeds and especially in the yeast plant. [Illustration: Fig. 106. Test for liberation of carbon dioxide from leafy plant during respiration. Baryta water in smaller vessel. (Sachs.)] =231. Energy set free during respiration.=—From what we have learned of the exchange of gases during respiration we infer that the plant loses carbon during this process. If the process of respiration is of any benefit to the plant, there must be some gain in some direction to compensate the plant for the loss of carbon which takes place. It can be shown by an experiment that during respiration there is a slight elevation of the temperature in the plant tissues. The plant then gains some heat during respiration. Energy is also manifested by growth. =232. Respiration in a leafy plant.=—We may take a potted plant which has a well-developed leaf surface and place it under a tightly fitting bell jar. Under the bell jar there also should be placed a small vessel containing baryta water. A similar apparatus should be set up, but with no plant, to serve as a check. The experiment must be set up in a room which is not frequented by persons, or the carbon dioxide in the room from respiration will vitiate the experiment. The bell jar containing the plant should be covered with a black cloth to prevent carbon assimilation. In the course of 10 or 12 hours, if everything has worked properly, the baryta water under the jar with the plant will show the film of barium carbonate, while the other one will show none. Respiration, therefore, takes place in a leafy plant as well as in germinating seeds. =233. Respiration in fungi.=—If several large actively growing mushrooms are accessible, place them in a tall glass jar as described for determining respiration in germinating peas. In the course of 12 hours test with the lighted taper and the baryta water. Respiration takes place in fungi as well as in green plants. =234. Respiration in plants in general.=—Respiration is general in all plants, though not universal. There are some exceptions in the lower plants, notably in certain of the bacteria, which can only grow and thrive in the absence of oxygen. [Illustration: Fig. 107. Fermentation tube with culture of yeast.]