Elements of Structural and Systematic Botany: For High Schools and Elementary College CoursesCampbell, Douglas Houghton
Science
Elements of Structural and Systematic Botany: For High Schools and Elementary College Courses
Campbell, Douglas Houghton
Botany -- Textbooks
There are, however, a few plants in which the ovules are very small
and transparent, so that they may be mounted whole and examined
alive. The best plant for this purpose is probably the "Indian pipe"
or "ghost flower," a curious plant growing in rich woods, blossoming
in late summer. It is a parasite or saprophyte, and entirely
destitute of chlorophyll, being pure white throughout. It bears a
single nodding flower at the summit of the stem. (Another species
much like it, but having several brownish flowers, is shown in
Figure 115, _L_.)
If this plant can be had, the structure of the ovule and embryo sac
may be easily studied, by simply stripping away the tissue bearing
the numerous minute ovules, and mounting a few of them in water, or
water to which a little sugar has been added.
[Illustration: FIG. 79.--_A_, ripe ovule of _Monotropa uniflora_, in
optical section, × 100. _m_, micropyle. _e_, embryo sac. _B_, the
embryo sac, × 300. At the top is the egg apparatus, consisting of the
two synergidæ (_s_), and the egg cell (_o_). In the centre is the
"endosperm nucleus" (_k_). At the bottom, the "antipodal cells" (_g_).]
The ovules are attached to a stalk, and each consists of about two
layers of colorless cells enclosing a central, large, oblong cell
(Fig. 79, _A_, _E_), the embryo sac or macrospore. If the ovule is
from a flower that has been open for some time, we shall find in the
centre of the embryo sac a large nucleus (_k_) (or possibly two
which afterward unite into one), and at each end three cells. Those
at the base (_g_) probably represent the prothallium, and those at
the upper end a very rudimentary archegonium, here generally called
the "egg apparatus."
Of the three cells of the "egg apparatus" the lower (_o_) one is the
egg cell; the others are called "synergidæ." The structure of the
embryo sac and ovules is quite constant among the angiosperms, the
differences being mainly in the shape of the ovules, and the degree
to which its coverings or integuments are developed.
The pollen spores of many angiosperms will germinate very easily in
a solution of common sugar in water: about fifteen per cent of sugar
is the best. A very good plant for this purpose is the sweet pea,
whose pollen germinates very rapidly, especially in warm weather.
The spores may be sown in a little of the sugar solution in any
convenient vessel, or in a hanging drop suspended in a moist
chamber, as described for germinating the spores of the slime
moulds. The tube begins to develop within a few minutes after the
spores are placed in the solution, and within an hour or so will
have reached a considerable length. Each spore has two nuclei, but
they are less evident here than in some other forms (Fig. 79).
[Illustration: FIG. 80.--Germinating pollen spores of the sweet pea,
× 200.]
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