of the equally hostile Clement VII. to recapture Reggio (1523) and Modena (1527), and was confirmed in his possession of them by the emperor Charles V., in spite of Clement's opposition. He died in 1534, and was succeeded by his son Ercole II. (1508-1559), who married Renee, daughter of Louis XII. of France, a princess of Protestant proclivities and a friend of Calvin. On joining the league of France and the papacy against Spain, Ercole was appointed lieutenant-general of the French army in Italy. The war was prosecuted, however, with little vigour, and peace was made with Spain in 1558. The duke and his brother, Cardinal Ippolito the Younger, were patrons of literature and art, and the latter built the magnificent Villa d' Este at Tivoli. He was succeeded by Alphonso II. (1533-1597), remembered for his patronage of Tasso, whom he afterwards imprisoned. He reorganized the army, enriched the public library, encouraged agriculture, but was extravagant and dissipated. With him the main branch of the family came to an end, and although at his death he bequeathed the duchy to his cousin Cesare (1533-1628), Pope Clement VIII., renewing the Church's hostility to the house of Este, declared that prince to be of illegitimate birth (a doubtful contention), and by a treaty with Lucrezia, Alphonso's sister, Ferrara was made over to the Holy See. Cesare held Modena and Reggio, but with him the Estensi cease to play an important part in Italian politics. For two centuries this dynasty had been one of the greatest powers in Italy, and its court was perhaps the most splendid in Europe, both as regards pomp and luxury and on account of the eminent artists, poets and scholars which it attracted.